Anticorps Recombinant de lapin anti-COL6A3

COL6A3 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

230540A11

N° de cat : 83010-1-RR

Synonymes

COL6A3-Specific, 230540A11, Collagen alpha 3(VI) chain, Collagen alpha-3(VI) chain, Collagen Type VI

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Galerie de données de validation

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  WB analysis of A375 using 83010-1-RR

  A375 cells were subjected to SDS PAGE followed by western blot with 83010-1-RR (COL6A3-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 83010-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug COL6A3-Specific Recombinant antibody (83010-1-RR, Clone:230540A11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83010-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83010-1-RR against Human COL6A3-Specific were performed. The affinity constant is 1.63 nM.

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Applications testées

• Résultats positifs en WB: cellules A375,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83010-1-RR cible COL6A3 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: COL6A3 Protéine recombinante Ag34752
• Nom complet: collagen, type VI, alpha 3
• Masse moléculaire calculée: 344 kDa
• Poids moléculaire observé: 300 kDa
• Numéro d’acquisition GenBank: NM_004369
• Symbole du gène: COL6A3
• Identification du gène (NCBI): 1293
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

COL6A3 belongs to the type VI collagen family. Collagen VI acts as a cell-binding protein. It is expected to be expressed in smooth muscle. And the calculated molecular weight of COL6A3 is 343 kDa. The protein has similar expression in obese and T2DM (2 patients, and it regulates the chemotaxis and inflammation of macrophages in adipose tissue. Its expression is also related to weight gain. The expression of this protein in adipocytes is related to insulin resistance, which is believed to depend on PPAR (peroxisome proliferator-activated receptor) α-mediated adipocyte development (PMID: 25337653). Defects in COL6A3 are a cause of Bethlem myopathy (BM). Defects in COL6A3 are a cause of Ullrich congenital muscular dystrophy (UCMD) which also known as Ullrich scleroatonic muscular dystrophy. This antibody is specific to COL6A3.

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