Anticorps Polyclonal de lapin anti-Coilin

Coilin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

16

Avis (1)

N° de cat : 10967-1-AP

Synonymes

COIL, p80-coilin, p80 coilin, CLN80

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Galerie de données de validation

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  WB analysis using 10967-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10967-1-AP (Coilin antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10967-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10967-1-AP (Coilin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 10967-1-AP

  IP result of anti-Coilin (IP:10967-1-AP, 4ug; Detection:10967-1-AP 1:10000) with HEK-293 cells lysate 1400 ug.

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  IHC staining of human prostate cancer using 10967-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10967-1-AP (Coilin antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 10967-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10967-1-AP (Coilin antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 10967-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10967-1-AP (Coilin antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 10967-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Coilin antibody (10967-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HEK-293 using 10967-1-AP

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human Coilin (10967-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules Jurkat, cellules K-562, tissu cérébral humain
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 14 publications below
• CoIP: See 2 publications below

Informations sur le produit

10967-1-AP cible Coilin dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Coilin Protéine recombinante Ag1416
• Nom complet: coilin
• Masse moléculaire calculée: 576 aa, 63 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: BC010385
• Symbole du gène: COIL
• Identification du gène (NCBI): 8161
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The nuclear coiled body(CBs) and nuclear structures known as gems contain high concentrations of the survival motor neurons (SMN) protein complex. CBs and gems often colocalize, and communication between them is mediated by COIL. COIL is a component of the CBS which are involved in the function or assembly/disassembly of nucleoplasmic snRNPs. During mitosis, CBs disassemble, coinciding with a mitotic-specific phosphorylation of p80 coilin, and symmetrical dimethylation of the COIL RG box motif is a molecular switch that determines whether the SMN complex forms gems or becomes incorporated into CBs

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; mRNAs are sorted for export or degradation before passing through nuclear speckles.; Authors - Jing Fan
mouse	WB,IHC,IF,CoIP	Clin Transl Med; HSF4/COIL complex-dependent R-loop mediates ultraviolet-induced inflammatory skin injury; Authors - Yi-Qian Feng
human	IF	PLoS Genet; Nuclear Fragile X Mental Retardation Protein Is localized to Cajal Bodies.; Authors - Dury Alain Y AY
human	IF	Elife; Characterisation of the biflavonoid hinokiflavone as a pre-mRNA splicing modulator that inhibits SENP.; Authors - Andrea Pawellek
human	WB,IF	Neurobiol Dis; UsnRNP trafficking is regulated by stress granules and compromised by mutant ALS proteins.; Authors - Simona Rossi
human	WB,IF	Mol Brain; Long non-coding RNA lncC11orf54-1 modulates neuroinflammatory responses by activating NF-κB signaling during meningitic Escherichia coli infection; Authors - Bojie Xu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Baptiste (Verified Customer) (09-05-2019)

This antibody works well in western blot

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: human satelllite muscle cells

Coilin Antibody Western Blot, validation (1:1000 dilution) in human satelllite muscle cells (Cat no:10967-1-AP)