Anticorps Polyclonal de lapin anti-CNPase

CNPase Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications

16

N° de cat : 13427-1-AP

Synonymes

CNP, EC:3.1.4.37, 2',3'-cyclic-nucleotide 3'-phosphodiesterase, 2',3' cyclic nucleotide 3' phosphodiesterase

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Galerie de données de validation

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  WB analysis using 13427-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13427-1-AP (CNPase antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 13427-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13427-1-AP (CNPase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 13427-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13427-1-AP (CNPase antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse brain using 13427-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13427-1-AP (CNPase antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of mouse brain using 13427-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 13427-1-AP (CNPase antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 13427-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 13427-1-AP (CNPase antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 13427-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human CNPase (13427-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IHC: See 8 publications below
• IF: See 4 publications below

Informations sur le produit

13427-1-AP cible CNPase dans les applications de WB, IHC, IF-P, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CNPase Protéine recombinante Ag4227
• Nom complet: 2',3'-cyclic nucleotide 3' phosphodiesterase
• Masse moléculaire calculée: 421 aa, 48 kDa
• Poids moléculaire observé: 45-48 kDa
• Numéro d’acquisition GenBank: BC028040
• Symbole du gène: CNPase
• Identification du gène (NCBI): 1267
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CNP(2',3'-cyclic-nucleotide 3'-phosphodiesterase) belongs to the cyclic nucleotide phosphodiesterase family. It plays an essential role in axonal survival but not in myelin assembly, coupling oligodendroglial functions in axonal support and myelination. This protein has 2 isoforms produced by alternative splicing with molecular mass of 45 and 48 kDa.

Publications

Species	Application	Title
mouse	IF	Nat Neurosci; TREM2 interacts with TDP-43 and mediates microglial neuroprotection against TDP-43-related neurodegeneration.; Authors - Manling Xie
mouse	IF	J Clin Invest; Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.; Authors - Zhixiong Liu
mouse	WB,IHC	J Pineal Res; Circadian misalignment impairs oligodendrocyte myelination via Bmal1 overexpression leading to anxiety and depression-like behaviors; Authors - Yao Zuo
mouse	WB	Acta Physiol (Oxf); Keratin 1 attenuates hypoxic pulmonary artery hypertension by suppressing pulmonary artery media smooth muscle expansion.; Authors - Li Zhang
mouse	IHC	Mol Nutr Food Res; A High MCT-Based Ketogenic Diet Suppresses Th1 and Th17 Responses to Ameliorate Experimental Autoimmune Encephalomyelitis in Mice by Inhibiting GSDMD and JAK2-STAT3/4 Pathways; Authors - Qianye Zhang
rat	IHC,IF	Front Cell Neurosci; Neural Stem Cells Overexpressing Nerve Growth Factor Improve Functional Recovery in Rats Following Spinal Cord Injury via Modulating Microenvironment and Enhancing Endogenous Neurogenesis.; Authors - Lei Wang