Anticorps Polyclonal de lapin anti-CNOT7

• Phare
• Validé par KD/KO

CNOT7 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications

6

Avis (1)

N° de cat : 14102-1-AP

Synonymes

BTG1 binding factor 1, CAF 1, CAF1, CCR4 associated factor 1, CNOT7, hCAF 1

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Galerie de données de validation

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  WB analysis of mouse testis using 14102-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14102-1-AP (CNOT7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 14102-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14102-1-AP (CNOT7 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 14102-1-AP

  IP result of anti-CNOT7 (IP:14102-1-AP, 4ug; Detection:14102-1-AP 1:300) with mouse testis tissue lysate 4800ug.

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris
• Résultats positifs en IP: tissu testiculaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IP: See 1 publications below

Informations sur le produit

14102-1-AP cible CNOT7 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CNOT7 Protéine recombinante Ag5235
• Nom complet: CCR4-NOT transcription complex, subunit 7
• Masse moléculaire calculée: 285 aa, 33 kDa
• Poids moléculaire observé: 33-35 kDa
• Numéro d’acquisition GenBank: BC060852
• Symbole du gène: CNOT7
• Identification du gène (NCBI): 29883
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cnot7, Ccr4-Not complex 7, is a component of CCR4-NOT complex and known to be a transcription factor and a modulator of mRNA degradation in yeast. Mammalian Cnot7 interacts with Tob and its family members, which is known as an inhibitor of BMP signaling through interfering with the Smad system and is involved in bone metabolism [PMID:17451368]. It is one of at least 8 subunits that make up the CCR4-Not transcription complex, which is involved in negative regulation of the pol II directed transcription in yeast24 and may be involved inglobal gene regulation by modulation of TATAA-binding protein (TBP) activity [PMID:10880468].

Publications

Species	Application	Title
human	WB	Nature; DND1 maintains germline stem cells via recruitment of the CCR4-NOT complex to target mRNAs.; Authors - Masashi Yamaji
human	IP	Nat Commun; TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV.; Authors - Roy Matkovic
human	WB	J Inflamm (Lond); The functional characterization of phosphorylation of tristetraprolin at C-terminal NOT1-binding domain.; Authors - Hsin-Hui Hsieh ; KD Validated
		Int J Mol Med; B cell translocation gene 1 reduces the biological outcome of kidney cancer through induction of cell proliferation, cell cycle arrest, cell apoptosis and cell metastasis.; Authors - Guogui Sun
mouse	WB	Cells; Translation Efficiency and Degradation of ER-Associated mRNAs Modulated by ER-Anchored poly(A)-Specific Ribonuclease (PARN).; Authors - Tian-Li Duan
human	WB	Nucleic Acids Res; BESST: a novel LncRNA knockout strategy with less genome perturbance; Authors - Shikuan Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Roy (Verified Customer) (06-13-2024)

Works well to detect CNOT7 (validated through siRNA-mediated KD of CNOT7 - 1/1000 dilution with an overnight incubation at 4°C).

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa