- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-CNOT2
CNOT2 Polyclonal Antibody for WB, IF, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, souris et plus (1)
Applications
WB, IF/ICC, ELISA
Conjugaison
Non conjugué
N° de cat : 10313-1-AP
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | cellules NIH/3T3, cellules HEK-293 |
| Résultats positifs en IF/ICC | cellules HEK-293 |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| KD/KO | See 2 publications below |
| WB | See 8 publications below |
Informations sur le produit
10313-1-AP cible CNOT2 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris
| Réactivité | Humain, souris |
| Réactivité citée | Humain, porc, souris |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Polyclonal |
| Type | Anticorps |
| Immunogène | CNOT2 Protéine recombinante Ag0347 |
| Nom complet | CCR4-NOT transcription complex, subunit 2 |
| Masse moléculaire calculée | 60 kDa |
| Poids moléculaire observé | 60 kDa |
| Numéro d’acquisition GenBank | BC002597 |
| Symbole du gène | CNOT2 |
| Identification du gène (NCBI) | 4848 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par affinité contre l'antigène |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
The Ccr4-Not complex is evolutionarily conserved and controls gene expression both at the level of transcription initiation and of mRNA degradation. And this complex can regulate pol II transcription. Promoter targeting of CCR4-NOT transcription complex subunit 2 (CNOT2) results in strong repression of pol II transcription. CNOT2-mediated repression is sensitive to the HDAC (histone deacetylase) inhibitor, TSA (trichostatin A).
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for CNOT2 antibody 10313-1-AP | Download protocol |
| IF protocol for CNOT2 antibody 10313-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Stem Cells Cnot1, Cnot2, and Cnot3 maintain mouse and human ESC identity and inhibit extraembryonic differentiation.
| ||
RNA Biol Essential functions of the CNOT7/8 catalytic subunits of the CCR4-NOT complex in mRNA regulation and cell viability. | ||
Stem Cells Dev Identification of Ccr4-Not complex components as regulators of transition from partial to genuine induced pluripotent stem cells.
| ||
bioRxiv Cnot3 is required for male germ cell development and spermatogonial stem cell maintenance | ||
Methods Mol Biol Dissecting the Role of the Ccr4-Not Deadenylase Complex in Pluripotency and Differentiation |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Abe (Verified Customer) (04-25-2024) | Worked well for IF at 1:100 overnight at 4c in RPE-1 cells.
|
FH Ayse (Verified Customer) (06-08-2022) | Used for IF experiment (dilution 1/100) and WB experiment (1/2000). The antibody works well for the 2 kind of experiment. In few weeks, I will also test for IP.
 |
