Anticorps Polyclonal de lapin anti-CNDP2

• Phare
• Validé par KD/KO

CNDP2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

13

N° de cat : 14925-1-AP

Synonymes

CN2, CNDP dipeptidase 2, CNDP2, CPGL, HsT2298, PEPA, Peptidase A

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Galerie de données de validation

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  WB analysis using 14925-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14925-1-AP (CNDP2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 14925-1-AP

  IP result of anti-CNDP2 (IP:14925-1-AP, 4ug; Detection:14925-1-AP 1:1000) with mouse testis tissue lysate 4000 ug.

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  IHC staining of human testis using 14925-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14925-1-AP (CNDP2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 14925-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14925-1-AP (CNDP2 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of PC-3 using 14925-1-AP

  Immunofluorescent analysis of PC-3 cells using 14925-1-AP (CNDP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules LNCaP, tissu rénal de souris
• Résultats positifs en IP: tissu testiculaire de souris,
• Résultats positifs en IHC: tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 12 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

14925-1-AP cible CNDP2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CNDP2 Protéine recombinante Ag6684
• Nom complet: CNDP dipeptidase 2 (metallopeptidase M20 family)
• Masse moléculaire calculée: 53 kDa
• Poids moléculaire observé: 44-53 kDa
• Numéro d’acquisition GenBank: BC001375
• Symbole du gène: CNDP2
• Identification du gène (NCBI): 55748
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Carnosine dipeptidase II (CNDP2) catalyzes the peptide bond hydrolysis in dipeptides, displaying a non-redundant activity toward threonyl dipeptides. It has high dipeptidase activity toward cysteinyl glycine, an intermediate metabolite in glutathione metabolism (PubMed:19346245, PubMed:12473676). Metabolizes N-lactoyl-amino acids, both through hydrolysis to form lactic acid and amino acids, as well as through their formation by reverse proteolysis (PubMed:25964343). CNDP2 has 2 isoforms with a molecular weight of 53 and 44 kDa. The isoform 1 is ubiquitously expressed with higher levels in the kidney and liver (at protein level) and expressed in peripheral blood leukocytes (PubMed:12473676). It is also expressed in gastric mucosa and down-regulated in gastric cancer mucosal tissues (at the protein level) (PubMed:24395568). Isoform 2 is broadly expressed in fetal tissues and expressed in the adult liver and placenta. (PMID: 17121880).

Publications

Species	Application	Title
	IHC	Acta Neuropathol; The proteome of granulovacuolar degeneration and neurofibrillary tangles in Alzheimer's disease.; Authors - David C Hondius
mouse	WB	Cell Chem Biol; Family-wide Annotation of Enzymatic Pathways by Parallel In Vivo Metabolomics.; Authors - Joon T Kim ; KO Validated
human	WB,IHC	Mol Med; Underexpressed CNDP2 participates in gastric cancer growth inhibition through activating the MAPK signaling pathway.; Authors - Zhenwei Zhang
human	WB,IF	BMC Cancer; The relationship between pepsinogen C and gastric carcinogenesis: a transgene and population study; Authors - Ying E
human	WB	PLoS One; Quantitative proteomics approach to screening of potential diagnostic and therapeutic targets for laryngeal carcinoma.; Authors - Li Li
human	WB,IHC	BMC Gastroenterol; Up-regulation of CNDP2 facilitates the proliferation of colon cancer.; Authors - Conglong Xue ; KD Validated