Anticorps Polyclonal de lapin anti-CNBP

• Phare
• Validé par KD/KO

CNBP Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA, IF

Conjugaison

Non conjugué

Publications

4

N° de cat : 14717-1-AP

Synonymes

RNF163, PROMM, DM2, CNBP1, Cellular nucleic acid-binding protein

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Galerie de données de validation

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  WB analysis of Caco-2 using 14717-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 14717-1-AP (CNBP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 14717-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14717-1-AP (CNBP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 14717-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14717-1-AP (CNBP Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  WB analysis of HeLa using 14717-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14717-1-AP (CNBP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 14717-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14717-1-AP (CNBP Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 14717-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules Caco-2, cellules HeLa, cellules U-251, tissu cardiaque de souris, tissu testiculaire de souris
• Résultats positifs en IHC: tissu ovarien humain, tissu cardiaque humain, tissu cérébral humain, tissu cutané humain, tissu pulmonaire humain, tissu rénal humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 2 publications below

Informations sur le produit

14717-1-AP cible CNBP dans les applications de WB, IHC, ELISA, IF et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CNBP Protéine recombinante Ag6441
• Nom complet: CCHC-type zinc finger, nucleic acid binding protein
• Masse moléculaire calculée: 19 kDa
• Poids moléculaire observé: 20-25 kDa
• Numéro d’acquisition GenBank: BC000288
• Symbole du gène: CNBP
• Identification du gène (NCBI): 7555
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Single stranded DNA-binding protein, with specificity to the sterol regulatory element (SRE). Involved in sterol-mediated repression.

Publications

Species	Application	Title
human	WB	PLoS Biol; Phosphoglucomutase 1 inhibits hepatocellular carcinoma progression by regulating glucose trafficking.; Authors - Guang-Zhi Jin ; KD Validated
mouse	WB	Mol Cell Proteomics; Quantitative proteomics reveals the essential roles of STIM1 in the testicular cord formation in mouse testis.; Authors - Bo Zheng
mouse	WB,IF	iScience; Proteostasis regulated by testis-specific ribosomal protein RPL39L maintains mouse spermatogenesis.; Authors - Qianxing Zou
mouse	WB,IF	Reproduction; Cellular nucleic acid-binding protein is vital to testis development and spermatogenesis in mice.; Authors - Bo Zheng