Anticorps Polyclonal de lapin anti-CLSTN3

CLSTN3 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

porc, rat, souris et plus (1)

Applications

WB, IHC, IP, CoIP, ELISA, IF

Conjugaison

Non conjugué

Publications

5

N° de cat : 13302-1-AP

Synonymes

calsyntenin 3, Alc-beta, alcbeta, Alcadein-beta, Alcadein beta

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Galerie de données de validation

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  WB analysis using 13302-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13302-1-AP (CLSTN3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of pig brain using 13302-1-AP

  pig brain tissue were subjected to SDS PAGE followed by western blot with 13302-1-AP (CLSTN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 13302-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13302-1-AP (CLSTN3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 13302-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13302-1-AP (CLSTN3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de porc, tissu cérébral de rat
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

13302-1-AP cible CLSTN3 dans les applications de WB, IHC, IP, CoIP, ELISA, IF et montre une réactivité avec des échantillons porc, rat, souris

• Réactivité: porc, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CLSTN3 Protéine recombinante Ag4107
• Nom complet: calsyntenin 3
• Masse moléculaire calculée: 968 aa, 107 kDa
• Poids moléculaire observé: 110-130 kDa
• Numéro d’acquisition GenBank: BC039075
• Symbole du gène: CLSTN3
• Identification du gène (NCBI): 9746
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Calsyntenins, also called alcadeins, are cadherin superfamily proteins first identified as synaptic proteins (PMID: 11161476; 12498782). Calsyntenins are type I transmembrane proteins with extracellular domains containing two cadherin repeats and an LNS (laminin, neurexin, sex hormone-binding globulin) domain (PMID: 24613359). Calsyntenin-3 (CLSTN3, also known as alcadein-beta) is a synapse-organizing protein that promotes the development of synapses. It is a postsynaptic adhesion molecule that binds to presynaptic neurexins to mediate both excitatory and inhibitory synapse formation (PMID: 25352602; 24613359).

Publications

Species	Application	Title
mouse	WB,IP	Nature; CLSTN3β enforces adipocyte multilocularity to facilitate lipid utilization; Authors - Kevin Qian
human,mouse	WB,IF	Mol Metab; CLSTN3 gene variant associates with obesity risk and contributes to dysfunction in white adipose tissue.; Authors - Ningning Bai
human	WB,CoIP	Int J Biol Macromol; Calsyntenin-3 interacts with the sodium-dependent vitamin C transporter-2 to regulate vitamin C uptake; Authors - Veedamali S. Subramanian
mouse	WB	Neuroscience; CircAFF2 promotes neuronal cell injury in intracerebral hemorrhage by regulating the miR-488/calsyntenin-3 axis; Authors - Juxing Qi ; KD Validated
mouse	WB	Int J Mol Sci; Transcriptome Profile in the Mouse Brain of Hepatic Encephalopathy and Alzheimer’s Disease; Authors - Young-Kook Kim