Anticorps Polyclonal de lapin anti-CLIC3

CLIC3 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 15971-1-AP

Synonymes

CLIC3

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Galerie de données de validation

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  WB analysis using 15971-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15971-1-AP (CLIC3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 15971-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15971-1-AP (CLIC3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 15971-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15971-1-AP (CLIC3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 15971-1-AP

  Immunofluorescent analysis of MCF-7 cells, using CLIC3 antibody 15971-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules JAR, tissu placentaire humain, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IHC: tissu placentaire humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

15971-1-AP cible CLIC3 dans les applications de WB, IHC, IF/ICC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CLIC3 Protéine recombinante Ag8757
• Nom complet: chloride intracellular channel 3
• Masse moléculaire calculée: 236 aa, 26 kDa
• Poids moléculaire observé: 27-30 kDa
• Numéro d’acquisition GenBank: BC007012
• Symbole du gène: CLIC3
• Identification du gène (NCBI): 9022
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume (PMID: 9880541). CLIC3 (chloride intracellular channel protein 3) belongs to the chloride channel CLIC family which has seven members based on sequence homology to the p64 chloride channels of bovine kidney microsomes. CLIC proteins lack the membrane-spanning domains characteristic of channel proteins and are largely intracellular. CLIC3 is predominantly localized in the nucleus and has a high expression level in placenta (PMID: 9880541; 17027078). It associates with the C-terminal of ERK7 and may participate in cellular growth control.

Publications

Species	Application	Title
rat	IHC	PLoS One; Mechanisms that determine the internal environment of the developing brain: a transcriptomic, functional and ultrastructural approach.; Authors - Liddelow Shane A SA
human	WB	J Cancer; Analysis of Differentially Expressed Genes in a Chinese Cohort of Esophageal Squamous Cell Carcinoma.; Authors - Gang Liu
human,rat	WB	Front Cell Neurosci; Identification of immune-related biomarkers in peripheral blood of schizophrenia using bioinformatic methods and machine learning algorithms; Authors - Xiaoli Zhu
human	WB,IF,CoIP	Cell Death Dis; CLIC3 interacts with NAT10 to inhibit N4-acetylcytidine modification of p21 mRNA and promote bladder cancer progression; Authors - Yujun Shuai ; KD Validated

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