Anticorps Monoclonal anti-Claudin 18

Claudin 18 Monoclonal Antibody for IHC, IP, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(5)

CloneNo.

5G7F2

N° de cat : 66167-1-Ig

Synonymes

UNQ778/PRO1572, CLDN18, Claudin-18, Claudin18, 5G7F2

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Galerie de données de validation

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  IP experiment of mouse stomach using 66167-1-Ig

  IP result of anti-Claudin 18 (IP:66167-1-Ig, 3ug; Detection:66167-1-Ig 1:300) with mouse stomach tissue lysate 4000ug.

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  IHC staining of human pancreas cancer using 66167-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66167-1-Ig (Claudin 18 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 66167-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66167-1-Ig (Claudin 18 antibody at dilution of 1:1000.

• 

  IHC staining of human stomach using 66167-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66167-1-Ig (Claudin 18 antibody at dilution of 1:1000.

• 

  IHC staining of human pancreas cancer using 66167-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66167-1-Ig (Claudin 18 antibody at dilution of 1:600 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 66167-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66167-1-Ig (Claudin 18 antibody at dilution of 1:600 (under 40x lens).

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  IHC staining of human ovary tumor using 66167-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66167-1-Ig (Claudin 18 antibody at dilution of 1:1000.

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  IHC staining of human ovary tumor using 66167-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66167-1-Ig (Claudin 18 antibody at dilution of 1:1000.

• 

  IHC staining of human stomach using 66167-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66167-1-Ig (Claudin 18 antibody) at dilution of 1:600 (under 40x lens).

• 

  IHC staining of human stomach using 66167-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66167-1-Ig (Claudin 18 antibody at dilution of 1:600 (under 10x lens).

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Applications testées

• Résultats positifs en IP: tissu d'estomac de souris
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu de tumeur ovarienne humain, tissu d'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Informations sur le produit

66167-1-Ig cible Claudin 18 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Claudin 18 Protéine recombinante Ag15517
• Nom complet: claudin 18
• Masse moléculaire calculée: 261 aa, 28 kDa
• Poids moléculaire observé: 29 kDa
• Numéro d’acquisition GenBank: BC146668
• Symbole du gène: CLDN18
• Identification du gène (NCBI): 51208
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Claudin 18 (CLDN18) belongs to the large claudin family of proteins, which are tetraspan transmembrane proteins of tight junctions (PMID: 11585919; 18036336). Claudins exhibit specific patterns of expression in different tissues (PMID: 15447685). CLDN18 is specifically expressed in the stomach and lung. CLDN18 has two alternatively spliced variants, CLDN18.1 and CLDN18.2. CLDN18.2 is a highly selective gastric lineage marker that determines the gastric phenotype in a neoplastic condition, whereas CLDN18.1 is lung specific (PMID: 11585919; 21832145). Altered CLDN18 expression has been reported in various human malignancies, including gastric cancer, lung adenocarcinoma, and pancreatic neoplasm (PMID: 17459057; 22076167; 21832145). This antibody can recognize both CLDN18.1 and CLDN18.2.

Publications

Species	Application	Title
human,mouse	IHC,IF	Mucosal Immunol; Airway epithelium IgE-FcεRI Cross-Link Induces Epithelial Barrier Disruption in Severe T2-high Asthma; Authors - Chih-Ming Weng
	IF	bioRxiv; Combined PI3K and MAPK inhibition synergizes to suppress PDAC; Authors - Bailey A Bye
		World J Gastrointest Pathophysiol; Visualization of sphingolipids and phospholipids in the fundic gland mucosa of human stomach using imaging mass spectrometry.; Authors - Nobuya Kurabe
human	WB,IHC,IF	Am J Cancer Res; The small protein LINC01547-ORF inhibits colorectal cancer progression by regulating the CLDN18-FAK-AKT axis; Authors - Shuai Zhang
mouse	IF	Cancers (Basel); Combined Omipalisib and MAPK Inhibition Suppress PDAC Growth; Authors - Bailey A Bye

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