Anticorps Polyclonal de lapin anti-CLASP1

CLASP1 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Avis (1)

N° de cat : 20803-1-AP

Synonymes

CLASP1, CLIP associating protein 1, hOrbit1, KIAA0622, MAST1, Multiple asters homolog 1, Protein Orbit homolog 1

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Galerie de données de validation

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  WB analysis using 20803-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20803-1-AP (CLASP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 20803-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20803-1-AP (CLASP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 20803-1-AP

  IP result of anti-CLASP1 (IP:20803-1-AP, 4ug; Detection:20803-1-AP 1:2000) with HeLa cells lysate 1750 ug.

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  IHC staining of human testis using 20803-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20803-1-AP (CLASP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 20803-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20803-1-AP (CLASP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HeLa, cellules SH-SY5Y, tissu cérébral de rat
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu testiculaire humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

20803-1-AP cible CLASP1 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CLASP1 Protéine recombinante Ag14724
• Nom complet: cytoplasmic linker associated protein 1
• Masse moléculaire calculée: 1538 aa, 169 kDa
• Poids moléculaire observé: 162 kDa
• Numéro d’acquisition GenBank: BC112940
• Symbole du gène: CLASP1
• Identification du gène (NCBI): 23332
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CLASP1 is a member of CLASP family. Members of the CLASP family of proteins are involved in multiple MT-dependent processes, including Golgi complex organization and trafficking, kinetochore fiber dynamics and flux, mitotic spindle assembly, and the stabilization of MT plus ends near the cortex. CLASP proteins regulate MT dynamics by promoting MT rescue and suppressing MT catastrophe. Recent work showed that CLASP1 activity is required for proper positioning of the mitotic spindle in both the C. elegans embryo and mammalian cells. Recent work has also identified roles CLASP1 in the regulation of spindle positioning.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sammy (Verified Customer) (11-05-2023)

Excellent antibody for detection of CLASP1 via Western Blotting.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HUVEC

CLASP1 Antibody Western Blot validation (1:1000 dilution) in HUVEC (Cat no:20803-1-AP)