Anticorps Polyclonal de lapin anti-CKB-Specific

CKB-Specific Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 18713-1-AP

Synonymes

B CK, CKB, CKBB, CKB-Specific, Creatine kinase B chain, Creatine kinase B type, creatine kinase, brain

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Galerie de données de validation

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  WB analysis of HEK-293 using 18713-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18713-1-AP (CKB-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 18713-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 18713-1-AP (CKB-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 18713-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 18713-1-AP (CKB-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 18713-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18713-1-AP (CKB-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 18713-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18713-1-AP (CKB-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 18713-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 18713-1-AP (CKB-Specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 18713-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18713-1-AP (CKB-Specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 18713-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 18713-1-AP (CKB-Specific antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 18713-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 18713-1-AP (CKB-Specific antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human gliomas using 18713-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 18713-1-AP (CKB-Specific antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary tumor using 18713-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 18713-1-AP (CKB-Specific Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human ovary tumor using 18713-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 18713-1-AP (CKB-Specific Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HEK-293 using 18713-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 18713-1-AP (CKB-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules Y79, tissu de côlon de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu de gliome humain, tissu de muscle squelettique de souris, tissu de tumeur ovarienne humain, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

18713-1-AP cible CKB-Specific dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: creatine kinase, brain
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC001190
• Symbole du gène: CKB
• Identification du gène (NCBI): 1152
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CKBB, also named as B-CK and CKB, is a member of the ATP:guanido phosphotransferase protein family. It is a cytoplasmic enzyme involved in energy homeostasis. CKBB reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. CK isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. CK MB consists of a dimer of nonidentical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain. This antibody is specific to CKB.