Anticorps Polyclonal de lapin anti-CIB1

• Phare
• Validé par KD/KO

CIB1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 11823-1-AP

Synonymes

Calmyrin, CIB, CIB1, CIBP, DNA PKcs interacting protein, Kinase interacting protein, KIP, KIP1, PRKDCIP, SIP2 28, SNK interacting protein 2 28

---

Galerie de données de validation

• 

  WB analysis of human liver using 11823-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11823-1-AP (CIB1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11823-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11823-1-AP (CIB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 11823-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 11823-1-AP (CIB1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 11823-1-AP

  IP result of anti-CIB1 (IP:11823-1-AP, 4ug; Detection:11823-1-AP 1:300) with mouse testis tissue lysate 4000ug.

• 

  IHC staining of human breast cancer using 11823-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11823-1-AP (CIB1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 11823-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11823-1-AP (CIB1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 11823-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11823-1-AP (CIB1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of rat testis using 11823-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis using 11823-1-AP (CIB1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of rat testis using 11823-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis using 11823-1-AP (CIB1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A549 using 11823-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CIB1 antibody (11823-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: tissu hépatique humain, cellules HepG2, tissu testiculaire de rat
• Résultats positifs en IP: tissu testiculaire de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu testiculaire de rat, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

11823-1-AP cible CIB1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CIB1 Protéine recombinante Ag2391
• Nom complet: calcium and integrin binding 1 (calmyrin)
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC000846
• Symbole du gène: CIB1
• Identification du gène (NCBI): 10519
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CIB1(Calcium and integrin-binding protein 1) is also named as CIB, KIP, PRKDCIP. It is an EF-hand-containing protein that binds multiple effector proteins, including the platelet αIIbβ3 integrin and several serine/threonine kinases and potentially modulates their function. It regulates platelet aggregation in hemostasis through a specific interaction with the alphaIIb cytoplasmic domain of platelet integrin alphaIIbbeta3.

Publications

Species	Application	Title
human	IP	Cell Death Differ; CHIP-mediated CIB1 ubiquitination regulated epithelial-mesenchymal transition and tumor metastasis in lung adenocarcinoma.; Authors - Yuanqi Liu
human	WB,IF	PLoS Pathog; CIB1 Synergizes with EphrinA2 to Regulate Kaposi's Sarcoma-Associated Herpesvirus Macropinocytic Entry in Human Microvascular Dermal Endothelial Cells.; Authors - Chirosree Bandyopadhyay ; KD Validated
mouse	WB	FASEB J; Identification of calcium and integrin-binding protein 1 as a novel regulator of production of amyloid β peptide using CRISPR/Cas9-based screening system.; Authors - Yung Wen Chiu ; KO Validated
rat	WB	Sci Rep; PDI regulates seizure activity via NMDA receptor redox in rats.; Authors - Ji Yang Kim
human	WB, IF	J Virol; p130Cas Scaffolds the Signalosome to Direct Adaptor-Effector Crosstalk During Kaposi's Sarcoma-Associated Herpesvirus Trafficking in Human Microvascular Dermal Endothelial Cells.; Authors - Chirosree Bandyopadhyay ; KD Validated
mouse	WB	Front Mol Neurosci; Expression of genes encoding the calcium signalosome in cellular and transgenic models of Huntington's disease.; Authors - Magdalena Czeredys