Anticorps Polyclonal de lapin anti-CHRNA5

CHRNA5 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 13516-1-AP

Synonymes

CHRNA5, LNCR2, NACHRA5

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Galerie de données de validation

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  WB analysis of Jurkat using 13516-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13516-1-AP (CHRNA5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 13516-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13516-1-AP (CHRNA5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse stomach using 13516-1-AP

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 13516-1-AP (CHRNA5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse bladder using 13516-1-AP

  mouse bladder tissue were subjected to SDS PAGE followed by western blot with 13516-1-AP (CHRNA5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 13516-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 13516-1-AP (CHRNA5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules A431, cellules A549, tissu d'estomac de souris, tissu vésical de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

13516-1-AP cible CHRNA5 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CHRNA5 Protéine recombinante Ag4447
• Nom complet: cholinergic receptor, nicotinic, alpha 5
• Masse moléculaire calculée: 468 aa, 53 kDa
• Poids moléculaire observé: 53 kDa
• Numéro d’acquisition GenBank: BC033639
• Symbole du gène: CHRNA5
• Identification du gène (NCBI): 1138
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nicotinic acetylcholine receptors (nAChRs), such as CHRNA5, are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be (hetero)pentamers composed of homologous subunits. Variants in the CHRNA5 gene are associated with nicotine dependence and lung cancer risk.

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