Anticorps Polyclonal de lapin anti-CHPF N-terminal

• Phare
• Validé par KD/KO

CHPF N-terminal Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 23953-1-AP

Synonymes

Chondroitin sulfate synthase 2, CHPF, CHPF N-terminal, CHSY2, CSS2

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Galerie de données de validation

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  WB analysis of human placenta using 23953-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 23953-1-AP (CHPF N-terminal antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 23953-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 23953-1-AP (CHPF N-terminal antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 23953-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 23953-1-AP (CHPF N-terminal antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse heart using 23953-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 23953-1-AP (CHPF N-terminal antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 23953-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 23953-1-AP (CHPF N-terminal antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 23953-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 23953-1-AP (CHPF N-terminal antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 23953-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 23953-1-AP (CHPF N-terminal antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 23953-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 23953-1-AP (CHPF N-terminal antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 23953-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 23953-1-AP (CHPF N-terminal antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 23953-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 23953-1-AP (CHPF N-terminal antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 23953-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 23953-1-AP (CHPF N-terminal antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 23953-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 23953-1-AP (CHPF N-terminal antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu placentaire humain, cellules C6, tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu cardiaque de souris, tissu cardiaque humain, tissu de cancer du côlon humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

23953-1-AP cible CHPF N-terminal dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CHPF N-terminal Protéine recombinante Ag20819
• Nom complet: chondroitin polymerizing factor
• Masse moléculaire calculée: 85 kDa
• Poids moléculaire observé: 85-90 kDa, 65-70 kDa
• Numéro d’acquisition GenBank: BC023531
• Symbole du gène: CHPF
• Identification du gène (NCBI): 79586
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Cells; Targeting Chondroitin Sulfate Reduces Invasiveness of Glioma Cells by Suppressing CD44 and Integrin β1 Expression.; Authors - Yin-Hung Chu
human	IHC,WB	J Cell Commun Signal; Chondroitin polymerizing factor predicts a poor prognosis and promotes breast cancer progression via the upstream TGF-β1/SMAD3 and JNK axis activation; Authors - Qiang-Feng Pan
human	WB,IHC,IF	Aging (Albany NY); The HNF4A-CHPF pathway promotes proliferation and invasion through interactions with MAD1L1 in glioma; Authors - Haitao Luo ; KD Validated