Anticorps Polyclonal de lapin anti-CHFR

• Phare
• Validé par KD/KO

CHFR Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

6

Avis (1)

N° de cat : 12169-1-AP

Synonymes

CHFR, RING finger protein 196, RNF116, RNF196

---

Galerie de données de validation

• 

  WB analysis of HepG2 using 12169-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12169-1-AP (CHFR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of multi-cells/tissue using 12169-1-AP

  WB result of 12169-1-AP (CHFR antibody).

• 

  WB analysis of mouse brain using 12169-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12169-1-AP (CHFR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12169-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12169-1-AP (CHFR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SMMC-7721 using 12169-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 12169-1-AP (CHFR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12169-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12169-1-AP (CHFR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human endometrial cancer using 12169-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 12169-1-AP (CHFR antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human endometrial cancer using 12169-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 12169-1-AP (CHFR antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung cancer using 12169-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12169-1-AP (CHFR antibody) at dilution of 1:50 (under 10x lens).

---

Applications testées

• Résultats positifs en WB: cellules HepG2, multi-cellules/tissus
• Résultats positifs en IHC: tissu de cancer de l'endomètre humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 2 publications below

Informations sur le produit

12169-1-AP cible CHFR dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CHFR Protéine recombinante Ag2811
• Nom complet: checkpoint with forkhead and ring finger domains
• Masse moléculaire calculée: 664 aa, 73 kDa
• Poids moléculaire observé: 70-85 kDa
• Numéro d’acquisition GenBank: BC012072
• Symbole du gène: CHFR
• Identification du gène (NCBI): 55743
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The Checkpoint with forkhead and RING finger domains protein(CHFR) is a checkpoint protein that reportedly initiates a cell cycle delay in response to microtubule stress during prophase in mitosis and it has been hypothesized to be a tumor suppressor with a potential role as a biomarker for chemotherapeutic response to Taxol(PMID:17596595). It belongs to the CHFR family. Endogenous Chfr is ubiquitinated and phosphorylated in an MPF-dependent manner in mitotic Xenopus egg extracts(PMID:18504434). It has 5 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	IHC	Clin Cancer Res; Association of CHFR promoter methylation with disease recurrence in locally advanced colon cancer.; Authors - Tanaka Motofumi M
human	WB	Transl Oncol; Silencing of CHFR Sensitizes Gastric Carcinoma to PARP Inhibitor Treatment.; Authors - Yuan Li
human	IHC	J Biol Chem; CHFR protein regulates mitotic checkpoint by targeting PARP-1 protein for ubiquitination and degradation.; Authors - Kashima Lisa L
human	WB	Front Genet; Identification of a Novel Epigenetic Signature CHFR as a Potential Prognostic Gene Involved in Metastatic Clear Cell Renal Cell Carcinoma.; Authors - Xiangling Chen ; KD Validated
human	WB	Mol Med Rep; CHFR‑mediated epithelial‑to‑mesenchymal transition promotes metastasis in human breast cancer cells.; Authors - Guobin Jiang ; KD Validated
human,mouse	WB	Nat Commun; Ubiquitin ligase CHFR mediated degradation of VE-cadherin through ubiquitylation disrupts endothelial adherens junctions; Authors - Chinnaswamy Tiruppathi ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Iram (Verified Customer) (02-11-2022)

This antibody works for me great

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000 for western and 1:500 for IF
• Cell Tissue Type: Colon cancer cells