Anticorps Polyclonal de lapin anti-CHEK2

CHEK2 Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 13954-1-AP

Synonymes

CDS1, CHEK2, Chk2, HuCds1, LFS2, PP1425, RAD53



Applications testées

Résultats positifs en WBcellules HL-60, cellules HeLa
Résultats positifs en IPcellules HeLa,
Résultats positifs en IF/ICCcellules HepG2,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

13954-1-AP cible CHEK2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéerat, Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène CHEK2 Protéine recombinante Ag5065
Nom complet CHK2 checkpoint homolog (S. pombe)
Masse moléculaire calculée 61 kDa
Poids moléculaire observé 65 kDa
Numéro d’acquisition GenBankBC004207
Symbole du gène CHEK2
Identification du gène (NCBI) 11200
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Chk2(Checkpoint kinase 2) is also named as CDS1, CHEK2, RAD53 and belongs to the CHK2 subfamily. It is a protein kinase that is activated in response to DNA damage and involved in cell cycle arrest. It also mediates the induction of senescence in fibroblasts, but is dispensable for the induction of telomere dysfunction checkpoints at the stem and progenitor cell level(PMID:20577265). This protein can exsit as a dimer (PMID:19782031). Active forms of CHEK2 devoid of phosphorylation at Thr-68 can form unstable dimers(PMID:12386164). Defects in CHEK2 is a cause of susceptibility to breast cancer (BC)(PMID:21618645). It has 12 isoforms produced by alternative splicing.

Protocole

Product Specific Protocols
WB protocol for CHEK2 antibody 13954-1-APDownload protocol
IF protocol for CHEK2 antibody 13954-1-APDownload protocol
IP protocol for CHEK2 antibody 13954-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

G-quadruplex DNA structures in human stem cells and differentiation.

Authors - Katherine G Zyner
humanWB

Oncogene

TRIM24 is critical for the cellular response to DNA double-strand breaks through regulating the recruitment of MRN complex

Authors - Ya Wang
humanWB

Clin Transl Med

Single-cell profiling-guided combination therapy of c-Fos and histone deacetylase inhibitors in diffuse large B-cell lymphoma.

Authors - Luqiao Wang
ratWB

Int J Nanomedicine

TiO2 Nanoparticles Caused DNA Damage in Lung and Extra-Pulmonary Organs Through ROS-Activated FOXO3a Signaling Pathway After Intratracheal Administration in Rats.

Authors - Bin Han
ratWB

Ecotoxicol Environ Saf

Hexavalent chromium caused DNA damage repair and apoptosis via the PI3K/AKT/FOXO1 pathway triggered by oxidative stress in the lung of rat

Authors - Lixia Zhang
humanWB

Oncotarget

UBE2D3 gene overexpression increases radiosensitivity of EC109 esophageal cancer cells in vitro and in vivo.

Authors - Xiaojia Gao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Priya (Verified Customer) (01-17-2023)

I have used this antibody for human keratinocytes, cardiomyocytes, mouse skin and liver tissues

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Cardiomyoctyes, Keratinocytes, mouse Liver and skin