• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-CHD4

CHD4 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

N° de cat : 14173-1-AP

Synonymes

ATP dependent helicase CHD4, CHD 4, CHD4, Mi 2b, Mi2 BETA



Applications testées

Résultats positifs en WBcellules HEK-293, cellules HeLa
Résultats positifs en IPcellules HeLa,
Résultats positifs en IHCtissu de cancer du col de l'utérus humain,
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IF/ICCcellules HeLa, cellules A549

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochimie (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

14173-1-AP cible CHD4 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Réactivité citéeHumain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène CHD4 Protéine recombinante Ag5372
Nom complet chromodomain helicase DNA binding protein 4
Masse moléculaire calculée 1937 aa, 221 kDa
Poids moléculaire observé 260 kDa
Numéro d’acquisition GenBankBC038596
Symbole du gène CHD4
Identification du gène (NCBI) 1108
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Chromodomain helicase DNA-binding protein 4 (CHD4) is an ATP dependent chromatin remodeler and a major subunit of the repressive NuRD complex [PMID:9790534]. This complex plays key roles in transcriptional regulation and reorganization and maintenance of chromatin structure, as it targets pericentromeric heterochromatin and has recently been implicated in DNA damage repair, being rapidly recruited to DNA double strand breaks [PMID:17694084]. CHD4 is a novel BRIT1 binding partner that regulates the HR repair process, which is a critical pathway of repairing DNA double strand breaks and plays an essential role in maintaining genomic integrity [PMID:22219182]. The calculated molecular weight of CHD4 is 218 kDa, but modified CHD4 is about 250-280 kDa.

Protocole

Product Specific Protocols
WB protocol for CHD4 antibody 14173-1-APDownload protocol
IHC protocol for CHD4 antibody 14173-1-APDownload protocol
IF protocol for CHD4 antibody 14173-1-APDownload protocol
IP protocol for CHD4 antibody 14173-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

ZNF410 Uniquely Activates the NuRD Component CHD4 to Silence Fetal Hemoglobin Expression.

Authors - Xianjiang Lan

Leukemia

Runx1 repression by histone deacetylation is critical for Setbp1-induced mouse myeloid leukemia development.

Authors - B A Vishwakarma
humanChIP,CoIP

Cancer Res

CHD4 Promotes Breast Cancer Progression as a Coactivator of Hypoxia-Inducible Factors.

Authors - Yijie Wang
  • KD Validated
humanWB,ChIP

Arthritis Rheumatol

Functional Genomic Analysis of a RUNX3 Polymorphism Associated with Ankylosing Spondylitis.

Authors - Matteo Vecellio
humanWB,IF

Clin Epigenetics

CHD4/NuRD complex regulates complement gene expression and correlates with CD8 T cell infiltration in human hepatocellular carcinoma.

Authors - Simin Shao
  • KD Validated
humanWB,IHC

Cell Signal

CHD4 mediates SOX2 transcription through TRPS1 in luminal breast cancer

Authors - Jun Zhang
  • KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Xiaochen (Verified Customer) (07-31-2023)

It is very sensitive and accurate. I can get the sepecific bend easily.

  • Applications: Western Blot
CHD4 Antibody Western Blot validation ( dilution) in (Cat no:14173-1-AP)
FH

Christos (Verified Customer) (01-26-2023)

Application: IF Sample: Human Cell line (HeLa Kyoto) Fixation: Paraformaldehyde (PFA 4% in PHEM pH=6.9) at room temperature for 15 minutes Permeabilization: 0.1% Triton-X-100 in PBS at room temperature for 5 minutes Blocking step: BSA as blocking agent for 1 hour · Concentration: 5% BSA in PBS · Temperature: RT dilution of the primary antibody: 1/50 incubation time: overnight at 4°C DNA (blue), CHD4 (green) and α-tubulin (red)

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1/50
  • Cell Tissue Type: Human cervix carcinoma cells/ HeLe Kyoto
CHD4 Antibody Immunofluorescence validation (1/50 dilution) in Human cervix carcinoma cells/ HeLe Kyoto (Cat no:14173-1-AP)