Anticorps Polyclonal de lapin anti-CHD4

• Phare
• Validé par KD/KO

CHD4 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

9

Avis (2)

N° de cat : 14173-1-AP

Synonymes

ATP dependent helicase CHD4, CHD 4, CHD4, Mi 2b, Mi2 BETA

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Galerie de données de validation

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  WB analysis of HEK-293 using 14173-1-AP

  WB result of CHD4 antibody (14173-1-AP, 1:1000) with si-Control and si-CHD4 transfected HEK293 cells.

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  WB analysis using 14173-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14173-1-AP (CHD4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14173-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14173-1-AP (CHD4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14173-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14173-1-AP (CHD4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14173-1-AP

  IP result of anti-CHD4 (IP:14173-1-AP, 4ug; Detection:14173-1-AP 1:300) with HeLa cells lysate 2640 ug.

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  IP experiment of HeLa using 14173-1-AP

  IP result of anti-CHD4 (IP:14173-1-AP, 4ug; Detection:14173-1-AP 1:1000) with HeLa cells lysate 1880 ug.

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  IHC staining of human cervical cancer using 14173-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14173-1-AP (CHD4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 14173-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14173-1-AP (CHD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 14173-1-AP

  Immunofluorescent analysis of A549 cells, using CHD4 antibody 14173-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of HeLa using 14173-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CHD4 antibody (14173-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 2 publications below

Informations sur le produit

14173-1-AP cible CHD4 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CHD4 Protéine recombinante Ag5372
• Nom complet: chromodomain helicase DNA binding protein 4
• Masse moléculaire calculée: 1937 aa, 221 kDa
• Poids moléculaire observé: 260 kDa
• Numéro d’acquisition GenBank: BC038596
• Symbole du gène: CHD4
• Identification du gène (NCBI): 1108
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Chromodomain helicase DNA-binding protein 4 (CHD4) is an ATP dependent chromatin remodeler and a major subunit of the repressive NuRD complex [PMID:9790534]. This complex plays key roles in transcriptional regulation and reorganization and maintenance of chromatin structure, as it targets pericentromeric heterochromatin and has recently been implicated in DNA damage repair, being rapidly recruited to DNA double strand breaks [PMID:17694084]. CHD4 is a novel BRIT1 binding partner that regulates the HR repair process, which is a critical pathway of repairing DNA double strand breaks and plays an essential role in maintaining genomic integrity [PMID:22219182]. The calculated molecular weight of CHD4 is 218 kDa, but modified CHD4 is about 250-280 kDa.

Publications

Species	Application	Title
human	WB	Mol Cell; ZNF410 Uniquely Activates the NuRD Component CHD4 to Silence Fetal Hemoglobin Expression.; Authors - Xianjiang Lan
		Leukemia; Runx1 repression by histone deacetylation is critical for Setbp1-induced mouse myeloid leukemia development.; Authors - B A Vishwakarma
human	ChIP,CoIP	Cancer Res; CHD4 Promotes Breast Cancer Progression as a Coactivator of Hypoxia-Inducible Factors.; Authors - Yijie Wang ; KD Validated
human	WB,ChIP	Arthritis Rheumatol; Functional Genomic Analysis of a RUNX3 Polymorphism Associated with Ankylosing Spondylitis.; Authors - Matteo Vecellio
human	WB,IF	Clin Epigenetics; CHD4/NuRD complex regulates complement gene expression and correlates with CD8 T cell infiltration in human hepatocellular carcinoma.; Authors - Simin Shao ; KD Validated
human	WB,IHC	Cell Signal; CHD4 mediates SOX2 transcription through TRPS1 in luminal breast cancer; Authors - Jun Zhang ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Xiaochen (Verified Customer) (07-31-2023)

It is very sensitive and accurate. I can get the sepecific bend easily.

• Applications: Western Blot

CHD4 Antibody Western Blot validation ( dilution) in (Cat no:14173-1-AP)

FH

Christos (Verified Customer) (01-26-2023)

Application: IF Sample: Human Cell line (HeLa Kyoto) Fixation: Paraformaldehyde (PFA 4% in PHEM pH=6.9) at room temperature for 15 minutes Permeabilization: 0.1% Triton-X-100 in PBS at room temperature for 5 minutes Blocking step: BSA as blocking agent for 1 hour · Concentration: 5% BSA in PBS · Temperature: RT dilution of the primary antibody: 1/50 incubation time: overnight at 4°C DNA (blue), CHD4 (green) and α-tubulin (red)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/50
• Cell Tissue Type: Human cervix carcinoma cells/ HeLe Kyoto

CHD4 Antibody Immunofluorescence validation (1/50 dilution) in Human cervix carcinoma cells/ HeLe Kyoto (Cat no:14173-1-AP)