Anticorps Polyclonal de lapin anti-CHD3

CHD3 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 25335-1-AP

Synonymes

hZFH, EC:3.6.4.12, Chromodomain-helicase-DNA-binding protein 3, CHD-3, CHD 3

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Galerie de données de validation

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  WB analysis using 25335-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25335-1-AP (CHD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 25335-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25335-1-AP (CHD3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 25335-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25335-1-AP (CHD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 25335-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25335-1-AP (CHD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 25335-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25335-1-AP (CHD3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon using 25335-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25335-1-AP (CHD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 25335-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25335-1-AP (CHD3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 25335-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25335-1-AP (CHD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 25335-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25335-1-AP (CHD3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon using 25335-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25335-1-AP (CHD3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 25335-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 25335-1-AP (CHD3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 25335-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 25335-1-AP (CHD3 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HEK-293, tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

25335-1-AP cible CHD3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: chromodomain helicase DNA binding protein 3
• Masse moléculaire calculée: 227 kDa
• Poids moléculaire observé: 240 kDa
• Numéro d’acquisition GenBank: NM_005852
• Symbole du gène: CHD3
• Identification du gène (NCBI): 1107
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CHD3, also named as Mi2 ALPHA or Zinc finger helicase, is a 2000 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain, and two chromo domain. CHD3 localizes in the nucleus and cytoplasm. CHD3 is widely expressed. CHD3 is a component of the histone deacetylase NuRD complex, which participates in the remodeling of chromatin by deacetylating histones. CHD3 is required for anchoring centrosomal pericentric in both interphase and mitosis.

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; CARM1 hypermethylates the NuRD chromatin remodeling complex to promote cell cycle gene expression and breast cancer development; Authors - Xue Chen