Anticorps Monoclonal anti-CHCHD2

• Phare
• Validé par KD/KO

CHCHD2 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

8

CloneNo.

2G1G10

N° de cat : 66302-1-Ig

Synonymes

C7orf17, CHCHD2, NS2TP

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Galerie de données de validation

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  WB analysis using 66302-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66302-1-Ig (CHCHD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of HEK-293 using 66302-1-Ig

  WB result of CHCHD2 antibody (66302-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHCHD2 transfected HEK-293 cells.

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  WB analysis using 66302-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66302-1-Ig (CHCHD2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 66302-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66302-1-Ig (CHCHD2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66302-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66302-1-Ig (CHCHD2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66302-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66302-1-Ig (CHCHD2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66302-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66302-1-Ig(CHCHD2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules Jurkat, cellules K-562, cellules L02, cellules LNCaP, cellules SMMC-7721, tissu hépatique de porc, tissu hépatique de rat
• Résultats positifs en IHC: tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Informations sur le produit

66302-1-Ig cible CHCHD2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat

• Réactivité: Humain, porc, rat
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CHCHD2 Protéine recombinante Ag24219
• Nom complet: coiled-coil-helix-coiled-coil-helix domain containing 2
• Masse moléculaire calculée: 151 aa, 16 kDa
• Poids moléculaire observé: 17 kDa
• Numéro d’acquisition GenBank: BC003079
• Symbole du gène: CHCHD2
• Identification du gène (NCBI): 51142
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CHCHD2 is a widely expressed 17 kDa mitochondrion-localized protein. CHCHD2 contains a C-terminal CHCH (coiled-coil helix coiled-coil helix) domain. The function of the CHCH domain is not well understood, and the few characterized proteins that harbor this domain have diverse functions. Mutations in CHCHD2 gene have been reported in autosomal dominant Parkinson's disease (ADPD).

Publications

Species	Application	Title
human	IF,WB	Proc Natl Acad Sci U S A; A nucleolar long "non-coding" RNA encodes a novel protein that functions in response to stress; Authors - Shuang Feng
human	WB	Proc Natl Acad Sci U S A; Mitochondrial Nuclear Retrograde Regulator 1 (MNRR1) rescues the cellular phenotype of MELAS by inducing homeostatic mechanisms.; Authors - Siddhesh Aras
human	IHC,IF,WB	Acta Neuropathol Commun; Reduced erythrocytic CHCHD2 mRNA is associated with brain pathology of Parkinson's disease.; Authors - Xiaodan Liu
human	WB	Hum Mol Genet; Loss of CHCHD10-CHCHD2 complexes required for respiration underlies the pathogenicity of a CHCHD10 mutation in ALS.; Authors - Isabella R Straub ; KD Validated
human	WB	Hum Mol Genet; Loss of CHCHD2 and CHCHD10 activates OMA1 peptidase to disrupt mitochondrial cristae phenocopying patient mutations.; Authors - Yi-Ting Liu ; KO Validated
human	WB,IF,IHC	Hum Mol Genet; CHCHD2 accumulates in distressed mitochondria and facilitates oligomerization of CHCHD10.; Authors - Xiaoping Huang ; KO Validated