Anticorps Polyclonal de lapin anti-CHAF1A

• Phare
• Validé par KD/KO

CHAF1A Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IF, IHC, ChIP, ELISA

Conjugaison

Non conjugué

Publications

7

Avis (1)

N° de cat : 17037-1-AP

Synonymes

CAF, CAF 1, CAF 1 subunit A, CAF I 150 kDa subunit, CAF I p150, CAF1, CAF1B, CAF1P150, CHAF1A, hp150, P150

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Galerie de données de validation

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  WB analysis of Jurkat using 17037-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17037-1-AP (CHAF1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17037-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17037-1-AP (CHAF1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 17037-1-AP

  IP result of anti-CHAF1A (IP:17037-1-AP, 4ug; Detection:17037-1-AP 1:1000) with Jurkat cells lysate 2400ug.

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  IHC staining of human cervix using 17037-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervix tissue slide using 17037-1-AP (CHAF1A Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cervix using 17037-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervix tissue slide using 17037-1-AP (CHAF1A Antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HeLa
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu de col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

17037-1-AP cible CHAF1A dans les applications de WB, IP, IF, IHC, ChIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CHAF1A Protéine recombinante Ag10536
• Nom complet: chromatin assembly factor 1, subunit A (p150)
• Masse moléculaire calculée: 956 aa, 107 kDa
• Poids moléculaire observé: 150 kDa
• Numéro d’acquisition GenBank: BC067093
• Symbole du gène: CHAF1A
• Identification du gène (NCBI): 10036
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Chromatin assembly factor 1 (CAF1) is the only histone chaperone known to assemble histones H3 and H4 onto newly synthesized DNA both in vitro and in vivo [PMID:17065558]. The 938 amino acid multidomain p150 (CHAF1A) binds via its C-terminal third to p60, which is an essential step for nucleosome assembly because knocking down either subunit disrupts the activity [PMID:14519857]. In addition, CAF1 facilitates DNA synthesis depending on the binding of the N-terminal 31 residues of p150 to the proliferating cell nuclear antigen (PCNA), which acts as a sliding clamp to stimulate the processivity of DNA polymerase [PMID:10648606]. CHAF1A regulates the formation of heterochromatin in mammalian cells during replication and in plants it maintains the transcription of certain subsets of genes. Furthermore, CHAF1A exists in a chromatin-remodeling complex WINAC, which coactivates ligand-induced transactivation function of the vitamin D receptor [PMID:12837248]. CHAF1A protein exists some phosphorylation sites, which may affect its theoretical molecular weight when tested.And a 150 kDa band was recognized (PMID:27445493).

Publications

Species	Application	Title
mouse	WB	Nat Struct Mol Biol; Reprogramming of the histone H3.3 landscape in the early mouse embryo.; Authors - Takashi Ishiuchi
human	ChIP	EMBO J; Histone chaperone CAF-1 promotes HIV-1 latency by leading the formation of phase-separated suppressive nuclear bodies.; Authors - Xiancai Ma ; KO Validated
human	IF	Cancers (Basel); Human Ccr4 and Caf1 Deadenylases Regulate Proliferation and Tumorigenicity of Human Gastric Cancer Cells via Modulating Cell Cycle Progression.; Authors - Xiao-Hui Song ; KD Validated
mouse	WB	J Biol Chem; The histone chaperone Spt6 is required for activation-induced cytidine deaminase target determination through H3K4me3 regulation.; Authors - Nasim A Begum
human	IHC	Front Mol Biosci; An integrated analysis of prognostic mRNA signature in early- and progressive-stage gastric adenocarcinoma; Authors - Xiaoling Hong
human	WB	Biomedicines; Co-Expression of Chromatin Assembly Factor 1 Subunit A and Proliferating Cell Nuclear Antigen Is a Prognostic Biomarker of Esophageal Cancer; Authors - Bing Wen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hasan (Verified Customer) (05-17-2023)

HeLa cells were pre-extracted for 5 mins then fixed with 4% PFA for 15 mins. Blocking with 5% FBS for 40 min and primary antibody incubation overnight.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: HeLa cells

CHAF1A Antibody Immunofluorescence validation (1:300 dilution) in HeLa cells (Cat no:17037-1-AP)