Anticorps Polyclonal de lapin anti-CHAC1

• Phare
• Validé par KD/KO

CHAC1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris, Hamster et plus (1)

Applications

WB, IF, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

24

N° de cat : 15207-1-AP

Synonymes

Blocks Notch protein, Botch, CHAC1

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Galerie de données de validation

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  WB analysis using 15207-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15207-1-AP (CHAC1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 15207-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15207-1-AP (CHAC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15207-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15207-1-AP (CHAC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15207-1-AP

  WB result of CHAC1 antibody (15207-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHAC1 transfected HeLa cells.

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  IHC staining of human stomach using 15207-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 15207-1-AP (CHAC1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human stomach using 15207-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 15207-1-AP (CHAC1 antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules C6, cellules CHO, cellules RAW 264.7, cellules U-251
• Résultats positifs en IHC: tissu d'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 21 publications below
• IHC: See 4 publications below
• IF: See 4 publications below
• CoIP: See 1 publications below

Informations sur le produit

15207-1-AP cible CHAC1 dans les applications de WB, IF, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris, Hamster

• Réactivité: Humain, rat, souris, Hamster
• Réactivité citée: Humain, levure, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CHAC1 Protéine recombinante Ag7360
• Nom complet: ChaC, cation transport regulator homolog 1 (E. coli)
• Masse moléculaire calculée: 24 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC001847
• Symbole du gène: CHAC1
• Identification du gène (NCBI): 79094
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CHAC1(Cation transport regulator-like protein 1) was identified in a co-regulated group of genes enriched for components of the ATF4 (activating transcription factor 4) arm of the unfolded protein response pathway. CHAC1 is a proapoptotic ER stress protein downstream of the pancreatic EIF2α kinase-ATF4 pathway that appears to be important for human physiology and disease(PMID: 19109178). In addition, the study found that high CHAC1 expression is associated with a bad prognosis hinting that CHAC1 may have a possible prognostic significance in breast cancer(PMID: 35930144). The predicted molecular weight of CHAC1 is 24 kDa, and the molecular weight detected by Proteintech is 38 kDa.

Publications

Species	Application	Title
	WB	Proc Natl Acad Sci U S A; Consequences of poly(ethylene oxide) and poloxamer P188 on transcription in healthy and stressed myoblasts; Authors - Adelyn A Crabtree
mouse	WB	Cell Mol Gastroenterol Hepatol; Hepatic TGFβr1 Deficiency Attenuates Lipopolysaccharide/D-Galactosamine-Induced Acute Liver Failure Through Inhibiting GSK3β-Nrf2-Mediated Hepatocyte Apoptosis and Ferroptosis.; Authors - Sha Huang
human	WB	Free Radic Biol Med; High-fat diet aggravates colitis-associated carcinogenesis by evading ferroptosis in the ER stress-mediated pathway.; Authors - Xiaoli Zhang
human	WB	Lung; ETS1 Ameliorates Hyperoxia-Induced Bronchopulmonary Dysplasia in Mice by Activating Nrf2/HO-1 Mediated Ferroptosis; Authors - Min Yang
human	WB	Front Oncol; Development and validation of an endoplasmic reticulum stress-related molecular prognostic model for breast cancer; Authors - Pengyu Fan
human	IHC	J Cell Mol Med; Ferroptosis-related gene NOX4, CHAC1 and HIF1A are valid biomarkers for stomach adenocarcinoma.; Authors - Ruoxi Xiao