Anticorps Polyclonal de lapin anti-CGGBP1

• Phare
• Validé par KD/KO

CGGBP1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, chIP, ELISA, Dot Blot

Conjugaison

Non conjugué

Publications

7

N° de cat : 10716-1-AP

Synonymes

20 kDa CGG binding protein, CGG binding protein 1, CGGBP, CGGBP1, p20 CGGBP, p20 CGGBP DNA binding protein

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Galerie de données de validation

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  WB analysis using 10716-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10716-1-AP (CGGBP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 10716-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10716-1-AP (CGGBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 10716-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10716-1-AP (CGGBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 10716-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10716-1-AP (CGGBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 10716-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10716-1-AP (CGGBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 10716-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10716-1-AP (CGGBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HL-60, cellules Jurkat, cellules K-562, cellules Raji, tissu de thymus de rat, tissu de thymus de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• ChIP: See 1 publications below

Informations sur le produit

10716-1-AP cible CGGBP1 dans les applications de WB, IP, IF, IHC, chIP, ELISA, Dot Blot et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CGGBP1 Protéine recombinante Ag1129
• Nom complet: CGG triplet repeat binding protein 1
• Masse moléculaire calculée: 19 kDa
• Poids moléculaire observé: 19-23 kDa
• Numéro d’acquisition GenBank: BC005222
• Symbole du gène: CGGBP1
• Identification du gène (NCBI): 8545
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CGGBP1, also named as CGGBP, is a 20 kDa CGG-binding protein. It binds to nonmethylated 5'-d(CGG)(n)-3' trinucleotide repeats in the FMR1 promoter. CGGBP1 may play a role in regulating FMR1 promoter. It is a bona fide midbody protein required for normal abscission and mitosis in general.(PMID:20832400)

Publications

Species	Application	Title
human	IP	Cell Cycle; CGGBP1 phosphorylation constitutes a telomere-protection signal.; Authors - Umashankar Singh ; KD Validated
human	chIP	Cell Cycle; Growth signals employ CGGBP1 to suppress transcription of Alu-SINEs.; Authors - Prasoon Agarwal
human	WB,IP	Epigenetics Chromatin; CGGBP1 regulates CTCF occupancy at repeats.; Authors - Divyesh Patel
human	IF	Exp Cell Res; CGGBP1 is a nuclear and midbody protein regulating abscission.; Authors - Singh Umashankar U
human	WB,Dot Blot	BMC Cancer; An immunochemistry-based screen for chemical inhibitors of DNA-protein interactions and its application to human CGGBP1.; Authors - Manthan Patel
human	WB	BMC Genet; CGGBP1-regulated cytosine methylation at CTCF-binding motifs resists stochasticity.; Authors - Manthan Patel