Anticorps Polyclonal de lapin anti-CFTR

CFTR Polyclonal Antibody for IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

N° de cat : 20738-1-AP

Synonymes

EC:5.6.1.6, Channel conductance-controlling ATPase, CFTR/MRP, cAMP-dependent chloride channel, ATP-binding cassette sub-family C member 7



Applications testées

Résultats positifs en IHCtissu pulmonaire humain, tissu de cancer du sein humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IF/ICCcellules HepG2
Résultats positifs en FC (Intra)cellules HUVEC,

Dilution recommandée

ApplicationDilution
Immunohistochimie (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

20738-1-AP cible CFTR dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Réactivité citéeHumain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Peptide
Nom complet cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7)
Masse moléculaire calculée 168 kDa
Numéro d’acquisition GenBankNM_000492
Symbole du gène CFTR
Identification du gène (NCBI) 1080
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

      What is the human cytogenetic location of the CFTR gene?

7q31.2

 

What is the predicted molecular weight of CFTR? 

168 kDa

 

Where is CFTR expressed?

CFTR is mainly expressed in apical membranes of vertebrate epithelial cells.

 

What is the ion selectivity of CFTR?

CFTR is an anion-selective channel, responsible for regulating chloride and bicarbonate transport.

 

What is the process of CFTR trafficking?

The ER provides the first steps in CFTR folding quality control. CFTR can be degraded by the ER associated degradation (ERAD) pathway, or it is transported to the Golgi for maturation before being trafficked to the cell membrane.

 

What are the separate domains of CFTR?

CFTR consists of two transmembrane domains, two nucleotide binding domains, and a regulatory domain.

 

What regulates CFTR?

CFTR activity is ATP-dependent and is regulated by protein kinase A phosphorylation.

 

What is the role of CFTR?

CFTR functions as a channel for anions, regulating water and ionic homeostasis.

 

What mutations in CFTR leads to protein degradation?

Deletion of the regulatory domain or the phenylalanine 508 (F508del) results in an increase in CFTR degradation. This degradation then leads to a decrease of secretion product clearance and an increase in viscous mucosal buildup at the epithelial surface of affected organs, the primary symptom of cystic fibrosis. Mutations in CFTR can also lead congenital bilateral absence of vas deferens (CBAVD) or sperm abnormalities in men.

 

How does CFTR differ from other ATP-binding cassette proteins?

Unlike other proteins in the ABC family, CFTR functions as an ion channel.


PMIDs: 24513531, 24534272, 22966013, 22709980


Protocole

Product Specific Protocols
IHC protocol for CFTR antibody 20738-1-APDownload protocol
IF protocol for CFTR antibody 20738-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Elife

Gq activity- and β-arrestin-1 scaffolding-mediated ADGRG2/CFTR coupling are required for male fertility.

Authors - Dao-Lai Zhang
humanIF

PLoS One

Heterogeneous expression of CFTR in insulin-secreting β-cells of the normal human islet.

Authors - Mauricio Di Fulvio
humanWB

J Exp Clin Cancer Res

Oridonin promotes endoplasmic reticulum stress via TP53-repressed TCF4 transactivation in colorectal cancer

Authors - Fangyuan Zhou
humanIHC

Int Immunopharmacol

A novel transcriptomic signature associated with lymphovascular invasion predicts clinical outcomes, tumor microenvironment, and therapeutic response in lung adenocarcinoma

Authors - Xing Huang
mouseIF

Biochem Biophys Res Commun

Dapagliflozin administration to a mouse model of type 2 diabetes induces DNA methylation and gene expression changes in pancreatic islets

Authors - Aisha Yokoi
mouseWB

Acta Pharmacol Sin

Alpha lipoamide inhibits diabetic kidney fibrosis via improving mitochondrial function and regulating RXRα expression and activation

Authors - Hui-Fang Zhang