Anticorps Polyclonal de lapin anti-CFHR3

CFHR3 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(9)

N° de cat : 16583-1-AP

Synonymes

CFHL3, CFHR3, complement factor H related 3, DOWN16, FHR 3, FHR3, H factor like protein 3, HLF4

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Galerie de données de validation

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  WB analysis using 16583-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16583-1-AP (CFHR3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 16583-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 16583-1-AP (CFHR3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 16583-1-AP

  IP result of anti-CFHR3 (IP:16583-1-AP, 4ug; Detection:16583-1-AP 1:300) with HepG2 cells lysate 2400ug.

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  IHC staining of human liver using 16583-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16583-1-AP (CFHR3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 16583-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16583-1-AP (CFHR3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, tissu cardiaque humain, tissu hépatique de rat
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

16583-1-AP cible CFHR3 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CFHR3 Protéine recombinante Ag9963
• Nom complet: complement factor H-related 3
• Masse moléculaire calculée: 330 aa, 37 kDa
• Poids moléculaire observé: 45-56 kDa
• Numéro d’acquisition GenBank: BC058009
• Symbole du gène: CFHR3
• Identification du gène (NCBI): 10878
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CFHR3, also named as DOWN16, belongs to the complement factor H-related protein family. Expressed by the liver and secreted in plasma, human CFHR3 is composed of five short consensus repeats (SCRs), and it also has a 19-amino acid signal peptide and four N-linked glycosylation sites. It may be involved in complement regulation. Deletion of CFHR1 and CFHR3 genes was found to be associated with decreased risk of age-related macular degeneration (AMD), and with an increased risk of atypical hemolytic-uremic syndrome (aHUS).

Publications

Species	Application	Title
human	WB	Clin Cancer Res; Complement regulatory proteins CFHR1 and CFHR3 and patient response to anti-CD20 monoclonal antibody therapy.; Authors - Laura M Rogers
human	WB	Sci Rep; A haplotype in CFH family genes confers high risk of rare glomerular nephropathies.; Authors - Yin Ding
human	IF	Exp Eye Res; Quantitative analysis of hydroxyapatite-binding plasma proteins in genotyped individuals with late-stage age-related macular degeneration.; Authors - Swati Arya
human	WB	PLoS One; A novel quantitative hemolytic assay coupled with restriction fragment length polymorphisms analysis enabled early diagnosis of atypical hemolytic uremic syndrome and identified unique predisposing mutations in Japan.; Authors - Yoko Yoshida
human	WB,IHC	Bioengineered; A novel hypoxia-driven gene signature that can predict the prognosis of hepatocellular carcinoma.; Authors - Zhirui Zeng
human	WB	Mol Med Rep; Complement factor H‑related 3 overexpression affects hepatocellular carcinoma proliferation and apoptosis.; Authors - Hong Liu

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