Anticorps Polyclonal de lapin anti-CEP70

CEP70 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 16280-1-AP

Synonymes

p10-binding protein, BITE

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Galerie de données de validation

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  WB analysis of human placenta using 16280-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 16280-1-AP (CEP70 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 16280-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16280-1-AP (CEP70 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 16280-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16280-1-AP (CEP70 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary using 16280-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16280-1-AP (CEP70 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 16280-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16280-1-AP (CEP70 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 16280-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CEP70 antibody (16280-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu placentaire humain, cellules PC-3, tissu testiculaire de souris
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

16280-1-AP cible CEP70 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CEP70 Protéine recombinante Ag9365
• Nom complet: centrosomal protein 70kDa
• Masse moléculaire calculée: 597 aa, 70 kDa
• Poids moléculaire observé: 25-28 kDa
• Numéro d’acquisition GenBank: BC016050
• Symbole du gène: CEP70
• Identification du gène (NCBI): 80321
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CEP70, also named as Centrosomal protein of 70 kDa, is a 597 amino acid protein, which directly interacts with tubulin-gamma; this interaction determines centrosomal localization. CEP70 plays a role in the organization of both preexisting and nascent microtubules in interphase cells. During mitosis, CEP70 is required for the organization and orientation of the mitotic spindle. CEP70 exists some isoforms with MW 70, 67, 65 and 25 kDa.

Publications

Species	Application	Title
human,mouse	IF	Front Endocrinol (Lausanne); Identification of biallelic variations of CEP70 in patients with male infertility; Authors - Tiechao Ruan
human	WB,IP	Cell Insight; LUC7L3 is a downstream factor of SRSF1 and prevents genomic instability; Authors - Xiaqing Zhang