Anticorps Polyclonal de lapin anti-CEP135
CEP135 Polyclonal Antibody for WB, IF, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
canin, Humain et plus (1)
Applications
WB, IF/ICC, ELISA
Conjugaison
Non conjugué
N° de cat : 24428-1-AP
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | cellules HeLa, cellules K-562, cellules MG U-87, cellules U2OS |
| Résultats positifs en IF/ICC | cellules MDCK, |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:3000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| WB | See 2 publications below |
| IF | See 7 publications below |
Informations sur le produit
24428-1-AP cible CEP135 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons canin, Humain
| Réactivité | canin, Humain |
| Réactivité citée | Humain, souris |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Polyclonal |
| Type | Anticorps |
| Immunogène | CEP135 Protéine recombinante Ag19122 |
| Nom complet | centrosomal protein 135kDa |
| Masse moléculaire calculée | 1140 aa, 134 kDa |
| Poids moléculaire observé | 135 kDa |
| Numéro d’acquisition GenBank | BC136535 |
| Symbole du gène | CEP135 |
| Identification du gène (NCBI) | 9662 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par affinité contre l'antigène |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
CEP135 is a 135 kDa centrosomal protein that is present in a wide range of organisms. CEP135 is located at the centrosome throughout the cell cycle, and localization is independent of the microtubule network. It distributes throughout the centrosomal area in association with the electron-dense material surrounding centrioles.
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for CEP135 antibody 24428-1-AP | Download protocol |
| IF protocol for CEP135 antibody 24428-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Curr Biol SAS-6 Association with γ-Tubulin Ring Complex Is Required for Centriole Duplication in Human Cells. | ||
EMBO Rep ENKD1 promotes CP110 removal through competing with CEP97 to initiate ciliogenesis. | ||
Elife WDR90 is a centriolar microtubule wall protein important for centriole architecture integrity. | ||
Cells Mitotic Maturation Compensates for Premature Centrosome Splitting and PCM Loss in Human cep135 Knockout Cells. | ||
Mol Biol Cell The SON RNA splicing factor is required for intracellular trafficking structures that promote centriole assembly and ciliogenesis. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Elisa (Verified Customer) (01-25-2023) | RPE1 cells stained for Hoechst (DNA marker, in blue), Cep135 (in magenta) and g-Tubulin (pericentriolar matrix marker, in green). RPE1 cells were fixed in cold methanol for 10' at -20C. Cells were then rehydrated with PBS for 5'. Membrane permeabilization was then performed with 0.1% Triton + 0.1% Tween +0.01%SDS in PBS for 5'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at RT. Primary antibody was diluted in blocking buffer 1:200 and incubated for 1h at room temperature. Alexa-488-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature).
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