Anticorps Recombinant de lapin anti-CENPF

CENPF Recombinant Antibody for IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

242277G9

N° de cat : 84865-4-RR

Synonymes

242277G9, CENF, CENP-F, PRO1779

---

Galerie de données de validation

• 

  IF Staining of HeLa using 84865-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CENPF antibody (84865-4-RR, Clone: 242277G9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  IF Staining of A431 using 84865-4-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CENPF antibody (84865-4-RR, Clone: 242277G9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  Affinity and Kinetic Characterization of 84865-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84865-4-RR against Human CENPF were performed. The affinity constant is 11.1 nM.

---

Applications testées

• Résultats positifs en IF/ICC: cellules HeLa, cellules A431

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84865-4-RR cible CENPF dans les applications de IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CENPF Protéine recombinante Ag29665
• Nom complet: centromere protein F, 350/400ka (mitosin)
• Masse moléculaire calculée: 368 kDa
• Numéro d’acquisition GenBank: NM_016343
• Symbole du gène: CENPF
• Identification du gène (NCBI): 1063
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CENPF, also named as mitosin and centromere protein F (PMID: 22912832), belongs to centromere protein F family. During the cell cycle, CENPF localizes in multiple cellular structures including the nuclear envelope in late G2/early prophase and kinetochores throughout mitosis (PMID: 30198378). CENPF is required for kinetochore function and chromosome segregation in mitosis and required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1 (PMID: 17600710)(PMID: 7542657). It regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation (PMID: 26125848). The calculated molecular weight of CENPF is ~358 kDa and CENPF can be detected as ~367 kDa (PMID: 7542657).

{{ptg:RelatedPrimaryAntibodies}}