Anticorps Polyclonal de lapin anti-CEL

• Phare
• Validé par KD/KO

CEL Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 15384-1-AP

Synonymes

Bile salt-activated lipase, BAL, Bucelipase, BSSL, BSDL

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Galerie de données de validation

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  WB analysis of human liver using 15384-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15384-1-AP (CEL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 15384-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15384-1-AP (CEL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15384-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15384-1-AP (CEL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15384-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15384-1-AP (CEL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 15384-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15384-1-AP (CEL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 15384-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15384-1-AP (CEL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human pancreas cancer using 15384-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using CEL antibody (15384-1-AP) at dilution of 1:200 and TRITC-Goat anti-Rabbit IgG, CoraLite® Plus 647 Glucagon antibody (CL647-15954, Magenta), CoraLite®488 INS antibody (CL488-66198, Clone: 4B6A7, green).

• 

  IF Staining of human pancreas cancer using 15384-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using CEL antibody (15384-1-AP) at dilution of 1:200 and TRITC-Goat anti-Rabbit IgG, CoraLite® Plus 647 Glucagon antibody (CL647-15954, Magenta), CoraLite®488 INS antibody (CL488-66198, Clone: 4B6A7, green).

• 

  IF Staining of human pancreas cancer using 15384-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using CEL antibody (15384-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), INS antibody (66198-1-Ig, Clone: 4B6A7, red).

• 

  IF Staining of HeLa using 15384-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15384-1-AP (CEL antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu hépatique humain, cellules HeLa, tissu cardiaque de souris, tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du pancréas humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cancer du pancréas humain,
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Informations sur le produit

15384-1-AP cible CEL dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CEL Protéine recombinante Ag5262
• Nom complet: carboxyl ester lipase (bile salt-stimulated lipase)
• Masse moléculaire calculée: 79 kDa
• Poids moléculaire observé: 67 kDa
• Numéro d’acquisition GenBank: BC042510
• Symbole du gène: CEL
• Identification du gène (NCBI): 1056
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Bile salt-activated lipase, previously named cholesterol esterase or bile salt-stimulated (or dependent) lipase, is a 74 kDa lipolytic enzyme capable of hydrolyzing cholesteryl esters, tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramide(PMID:12454261). The same enzyme is present as a major protein in milk and as a minor constituent in liver, activated macrophages, and endothelial cells(PMID:11733511). Defects in CEL are a cause of maturity-onset diabetes of the young type 8 with exocrine dysfunction (MODY8)(PMID:16369531). The full length protein has 11 glycosylation sites. It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	IF	J Crohns Colitis; Exosomal miR-103a-3p from Crohn's creeping fat-derived ASCs contributes to intestinal fibrosis by targeting TGFBR3 and activating fibroblasts; Authors - Wenwei Qian
mouse	WB,IHC	Cell Mol Gastroenterol Hepatol; The CEL-HYB1 Hybrid Allele Promotes Digestive Enzyme Misfolding and Pancreatitis in Mice.; Authors - Xiao-Tong Mao
human	WB,IF	ACS Biomater Sci Eng; LINC01915 Facilitates the Conversion of Normal Fibroblasts into Cancer-Associated Fibroblasts Induced by Colorectal Cancer-Derived Extracellular Vesicles through the miR-92a-3p/KLF4/CH25H Axis.; Authors - Minghe Zhou
HUMAN	WB	Biochemistry; Branched Fatty Acid Esters of Hydroxy Fatty Acids Are Preferred Substrates of the MODY8 Protein Carboxyl Ester Lipase.; Authors - Matthew J Kolar ; KO Validated
human	IF,IHC,WB	Heliyon; Integrative analysis of cancer-associated fibroblast signature in gastric cancer; Authors - Zidan Zhao