Anticorps Polyclonal de lapin anti-CDX1

CDX1 Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 21655-1-AP

Synonymes

caudal type homeobox 1, Caudal type homeobox protein 1, CDX1, Homeobox protein CDX 1

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Galerie de données de validation

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  WB analysis using 21655-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 21655-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 21655-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse small intestine using 21655-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 21655-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse stomach using 21655-1-AP

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 21655-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 21655-1-AP (CDX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of Caco-2 using 21655-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using CDX1 antibody (21655-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules Caco-2, tissu de côlon de rat, tissu de côlon de souris, tissu d'estomac de souris, tissu d'intestin grêle de souris
• Résultats positifs en IF/ICC: cellules Caco-2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

21655-1-AP cible CDX1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CDX1 Protéine recombinante Ag16376
• Nom complet: caudal type homeobox 1
• Masse moléculaire calculée: 265 aa, 28 kDa
• Poids moléculaire observé: 28 kDa, 35 kDa
• Numéro d’acquisition GenBank: BC096252
• Symbole du gène: CDX1
• Identification du gène (NCBI): 1044
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.