Anticorps Polyclonal de lapin anti-CDK6

• Phare
• Validé par KD/KO

CDK6 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

15

N° de cat : 19117-1-AP

Synonymes

Serine/threonine-protein kinase PLSTIRE, EC:2.7.11.22, Cyclin-dependent kinase 6, Cyclin dependent kinase 6, Cell division protein kinase 6

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Galerie de données de validation

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  WB analysis using 19117-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19117-1-AP (CDK6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 19117-1-AP

  WB result of CDK6 antibody (19117-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDK6 transfected HeLa cells.

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  IP experiment of Jurkat using 19117-1-AP

  IP result of anti-CDK6 (IP:19117-1-AP, 4ug; Detection:19117-1-AP 1:1000) with Jurkat cells lysate 1960 ug.

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  IHC staining of human lung cancer using 19117-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19117-1-AP (CDK6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 19117-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19117-1-AP (CDK6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 19117-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 19117-1-AP (CDK6 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 19117-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 19117-1-AP (CDK6 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human endometrial cancer using 19117-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 19117-1-AP (CDK6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 19117-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 19117-1-AP (CDK6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 19117-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK6 antibody (19117-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  FC experiment of HeLa using 19117-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human CDK6 (19117-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules K-562
• Résultats positifs en IP: cellules Jurkat,
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer de l'endomètre humain, tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 15 publications below

Informations sur le produit

19117-1-AP cible CDK6 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CDK6 Protéine recombinante Ag5600
• Nom complet: cyclin-dependent kinase 6
• Masse moléculaire calculée: 326 aa, 36 kDa
• Poids moléculaire observé: 36-40 kDa
• Numéro d’acquisition GenBank: BC027989
• Symbole du gène: CDK6
• Identification du gène (NCBI): 1021
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CDK6(Cyclin-dependent kinase 6) is involved in initiation and maintenance of cell cycle exit during cell differentiation and it prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types.The molecular weight of CDK6 is 36-40 KDa (PMID: 23563707, 8114739).

Publications

Species	Application	Title
human	WB	Theranostics; Estrogen-induced circRNA, circPGR, functions as a ceRNA to promote estrogen receptor-positive breast cancer cell growth by regulating cell cycle-related genes.; Authors - Lei Wang
human	WB	Cancers (Basel); A Patient-Derived Xenograft Model of Dedifferentiated Endometrial Carcinoma: A Proof-of-Concept Study for the Identification of New Molecularly Informed Treatment Approaches.; Authors - Chiao-Yun Lin
human	WB	Oncotarget; High expression of N-myc (and STAT) interactor predicts poor prognosis and promotes tumor growth in human glioblastoma.; Authors - Delong Meng
human	WB	Front Pharmacol; Oxymatrine Inhibits Renal Cell Carcinoma Progression by Suppressing β-Catenin Expression.; Authors - Yinshan Jin
human	WB	Cancer Biother Radiopharm; γ-Glutamylcyclotransferase Knockdown Inhibits Growth of Lung Cancer Cells Through G0/G1 Phase Arrest.; Authors - Zhifeng Lin
human	WB	Cancer Med; δ-Catenin regulates proliferation and apoptosis in renal cell carcinoma via promoting β-catenin nuclear localization and activating its downstream target genes.; Authors - Lincheng Ju