Anticorps Polyclonal de lapin anti-CDK5

• Phare
• Validé par KD/KO

CDK5 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

13

Avis (2)

N° de cat : 10430-1-AP

Synonymes

CDK5, Cell division protein kinase 5, cyclin dependent kinase 5, PSSALRE, TPKII catalytic subunit

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Galerie de données de validation

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  WB analysis using 10430-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10430-1-AP (CDK5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 10430-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 10430-1-AP (CDK5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 10430-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10430-1-AP (CDK5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 10430-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10430-1-AP (CDK5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 10430-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10430-1-AP (CDK5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 10430-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10430-1-AP (CDK5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 10430-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10430-1-AP (CDK5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 10430-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10430-1-AP (CDK5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 10430-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10430-1-AP (CDK5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 10430-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CDK5 antibody (10430-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules NIH/3T3, tissu cérébral de souris, tissu pancréatique de souris
• Résultats positifs en IHC: tissu rénal de souris, tissu de carcinome à cellules rénales humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 13 publications below

Informations sur le produit

10430-1-AP cible CDK5 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CDK5 Protéine recombinante Ag0686
• Nom complet: cyclin-dependent kinase 5
• Masse moléculaire calculée: 31 kDa, 33 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC005115
• Symbole du gène: CDK5
• Identification du gène (NCBI): 1020
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cyclin-dependent kinase 5 (CDK5), belongs to the cyclin-dependent kinase family, is a proline-directed serine/threonine-protein kinase that essential for neuronal cell cycle arrest and differentiation and may be involved in apoptotic cell death in neuronal diseases by triggering abortive cell cycle re-entry. CDK5 predominantly expressed in neurons where it phosphorylates both high molecular weight neurofilaments and microtubule-associated protein tau.

Publications

Species	Application	Title
human	WB	Signal Transduct Target Ther; SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis; Authors - Saisai Guo
mouse	WB	Neuropharmacology; Inhibition of Cdk5 rejuvenates inhibitory circuits and restores experience-dependent plasticity in adult visual cortex.; Authors - Yue Li ; KD Validated
human	WB	Cells; Phosphorylation-Induced Ubiquitination and Degradation of PXR through CDK2-TRIM21 Axis.; Authors - Mengyao Qin ; KD Validated
human	WB	Am J Transl Res; Hypoxia induces HT-22 neuronal cell death via Orai1/CDK5 pathway-mediated Tau hyperphosphorylation.; Authors - Binbin Fang
mouse	WB	J Cell Biochem; Nestin protects mouse podocytes against high glucose-induced apoptosis by a Cdk5-dependent mechanism.; Authors - Liu Wei W
human,mouse	WB	BMC Neurol; The LPA-CDK5-tau pathway mediates neuronal injury in an in vitro model of ischemia-reperfusion insult.; Authors - Yaya Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

XinN (Verified Customer) (02-27-2023)

It can recognize endogenous CDK5 with a right MW.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse brain lysate

CDK5 Antibody Western Blot validation (1:2000 dilution) in mouse brain lysate (Cat no:10430-1-AP)

FH

Kathryn (Verified Customer) (04-22-2022)

This CDK5 antibody works very well to stain the nuclei of developed mouse oocytes.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse ovary sections

CDK5 Antibody Immunofluorescence validation (1:100 dilution) in Mouse ovary sections (Cat no:10430-1-AP)