Anticorps Polyclonal de lapin anti-CDCA4

• Phare
• Validé par KD/KO

CDCA4 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 11625-1-AP

Synonymes

CDCA4, HEPP, SEI 3/HEPP

---

Galerie de données de validation

• 

  WB analysis using 11625-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11625-1-AP (CDCA4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11625-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11625-1-AP (CDCA4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11625-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11625-1-AP (CDCA4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11625-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11625-1-AP (CDCA4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

---

Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules MCF-7, cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 6 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

11625-1-AP cible CDCA4 dans les applications de WB, IP, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CDCA4 Protéine recombinante Ag2214
• Nom complet: cell division cycle associated 4
• Masse moléculaire calculée: 241 aa, 26 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC025263
• Symbole du gène: CDCA4
• Identification du gène (NCBI): 55038
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRIP-Br proteins are a set of proteins with the potential to function in both transcriptional control and cell cycle regulation [PMID:18572021]. CECA4 is a member of TRIP-br family, based on its homologous sequence motifs including SERTA and a PHD-bromo-binding site which interacts with a series of bromodomain containing transcriptional cofactors[PMID:17141982]. When activated by E2F, CDCA4 consequently functions as a suppressor of E2F-responsive promoters to participate the regulation of cell proliferation through the E2F/pRb pathway [PMID:16098148].

Publications

Species	Application	Title
mouse	WB	Cancer Cell; The DLEU2/miR-15a/16-1 cluster controls B cell proliferation and its deletion leads to chronic lymphocytic leukemia.; Authors - Ulf Klein ; KD Validated
human	IF	Sci Rep; Hepatocellular Carcinoma-propagating Cells are Detectable by Side Population Analysis and Possess an Expression Profile Reflective of a Primitive Origin.; Authors - Honghai Xia
human	WB,CoIP	Cancer Cell Int; CDCA4 suppresses epithelial-mesenchymal transtion (EMT) and metastasis in Non-small cell lung cancer through modulating autophagy.; Authors - Chenxin Xu ; KD Validated
human	WB,IP	Thorac Cancer; CDCA4 interacts with IGF2BP1 to regulate lung adenocarcinoma proliferation via the PI3K/AKT pathway; Authors - Sitong Feng ; KD Validated
human,mouse	WB	Gene; ALKBH5 promotes the development of lung adenocarcinoma by regulating the polarization of M2 macrophages through CDCA4; Authors - Jianlong Tan ; KD Validated
human	WB	Front Mol Biosci; Construction of shared gene signature between rheumatoid arthritis and lung adenocarcinoma helps to predict the prognosis and tumor microenvironment of the LUAD patients; Authors - Liping Shi