Anticorps Recombinant de lapin anti-CD9

CD9 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

5K14

N° de cat : 82105-1-RR

Synonymes

CD9 antigen, BTCC 1, BA2, 5K14, 5H9 antigen

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Galerie de données de validation

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  WB analysis using 82105-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82105-1-RR (CD9 antibody) at dilution of 1:24370 incubated at room temperature for 1.5 hours.

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  WB analysis of human urine exosomes using 82105-1-RR

  human urine and human urine-derived exosomes were subjected to SDS PAGE followed by western blot with 82105-1-RR (CD9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 82105-1-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82105-1-RR (CD9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 82105-1-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82105-1-RR (CD9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 82105-1-RR

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 82105-1-RR (CD9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 82105-1-RR

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 82105-1-RR (CD9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 82105-1-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CD9 antibody (82105-1-RR, Clone: 5K14 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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  Affinity and Kinetic Characterization of 82105-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82105-1-RR against Human CD9 were performed. The affinity constant is 36.3 pM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431, cellules L02
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer de l'endomètre humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:650-1:2600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

82105-1-RR cible CD9 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD9 Protéine recombinante Ag14546
• Nom complet: CD9 molecule
• Masse moléculaire calculée: 228 aa, 25 kDa
• Poids moléculaire observé: 23-27 kDa
• Numéro d’acquisition GenBank: BC011988
• Symbole du gène: CD9
• Identification du gène (NCBI): 928
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD9, also known as Tspan-29, p24 or MIC3, is a member of the tetraspanin superfamily (PMID: 1879540). It is expressed on a large variety of hematopoietic and non-hematopoietic cells, such as stromal cells, megakaryocytes, platelets, B and T lymphocytes, dendritic cells, endothelial cells, mast cells, eosinophils, and basophils (PMID: 30356731). CD9 interacts with the integrin family and other membrane proteins, and is involved in cell adhesion, cell motility and tumor metastasis (PMID: 8478605; 21428940; 25805926). Expression of CD9 enhances membrane fusion between muscle cells and support myotube maintenance (PMID:10459022). On oocytes, CD9 is hypothesized to play a role in fertilization of mammals (PMID: 25536312).

Publications

Species	Application	Title
human	WB	J Control Release; 3D mesenchymal stem cell exosome-functionalized hydrogels for corneal wound healing; Authors - Yuehe Xu

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