Anticorps Monoclonal anti-CD80 (B7-1)

CD80 (B7-1) Monoclonal Antibody for FC

Hôte / Isotype

Armenian Hamster / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

16-10A1

N° de cat : CL488-65076

Synonymes

B7 1, B7.1, Cd28l, Cd80, CD80 (B7-1), CD80 antigen, Ly 53, Ly53, MIC17, TS/A 1

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Galerie de données de validation

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  FC experiment of BALB/C mouse peritoneal macrophages using CL488-65076

  1X10^6 BALB/C mouse peritoneal macrophages were surface stained with 0.5 ug CoraLite® Plus 488 Anti-Mouse CD80 (B7-1) (CL488-65076, Clone:16-10A1), or unstained. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: macrophages péritonéaux de souris Balb/c,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-65076 cible CD80 (B7-1) dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Armenian Hamster / IgG
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lignée de cellules CHO transfectées avec B7 (CD80) de souris
• Nom complet: CD80 antigen
• Numéro d’acquisition GenBank: BC131959
• Symbole du gène: Cd80
• Identification du gène (NCBI): 12519
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)