Anticorps Polyclonal de lapin anti-CD68

CD68 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 30929-1-AP

Synonymes

CD68 antigen, gp110, Scard1

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Galerie de données de validation

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  WB analysis using 30929-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30929-1-AP (CD68 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 30929-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 30929-1-AP (CD68 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 30929-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 30929-1-AP (CD68 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 30929-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 30929-1-AP (CD68 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 30929-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 30929-1-AP (CD68 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat spleen using 30929-1-AP

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 30929-1-AP (CD68 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat spleen using 30929-1-AP

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 30929-1-AP (CD68 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 30929-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30929-1-AP (CD68 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 30929-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30929-1-AP (CD68 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse spleen using 30929-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD68 antibody (30929-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse spleen using 30929-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD68 antibody (30929-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: NR8383 cells, cellules RAW 264.7
• Résultats positifs en IHC: tissu splénique de souris, tissu hépatique de rat, tissu hépatique de souris, tissu splénique de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu splénique de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below

Informations sur le produit

30929-1-AP cible CD68 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD68 Protéine recombinante Eg0787
• Nom complet: CD68 antigen
• Masse moléculaire calculée: 35 kDa
• Poids moléculaire observé: 90-100 kDa
• Numéro d’acquisition GenBank: NM_001291058.1
• Symbole du gène: Cd68
• Identification du gène (NCBI): 12514
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mouse CD68 (also known as macrosialin) is a type I transmembrane glycoprotein that is highly and specifically expressed by mouse tissue macrophages, and to a lesser extent by dendritic cells. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

Publications

Species	Application	Title
mouse	IHC	J Nanobiotechnology; Biomimetic mesenchymal stem cell membrane-coated nanoparticle delivery of MKP5 inhibits hepatic fibrosis through the IRE/XBP1 pathway; Authors - Yafei Tian

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