Anticorps Monoclonal anti-CD43

CD43 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

CloneNo.

2A11D6

N° de cat : 66224-1-Ig

Synonymes

CD43, Galactoglycoprotein, GALGP, GPL115, Leukocyte sialoglycoprotein, Leukosialin, LSN, sialophorin, SPN

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Galerie de données de validation

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  WB analysis of Jurkat using 66224-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66224-1-Ig (CD43 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 66224-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 66224-1-Ig (CD43 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 66224-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66224-1-Ig (CD43 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66224-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66224-1-Ig (CD43 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 66224-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66224-1-Ig (CD43 antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 66224-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66224-1-Ig (CD43 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 66224-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66224-1-Ig (CD43 antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 66224-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66224-1-Ig (CD43 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Jurkat using 66224-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using 66224-1-Ig (CD43 antibody) at dilution of 1:400 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human tonsillitis using 66224-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66224-1-Ig (CD43 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of Jurkat using 66224-1-Ig

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human CD43 (66224-1-Ig, Clone:2A11D6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules K-562
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu d'appendicite humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules Jurkat, tissu d'amygdalite humain
• Résultats positifs en cytométrie: cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:4000-1:16000
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66224-1-Ig cible CD43 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD43 Protéine recombinante Ag23595
• Nom complet: sialophorin
• Masse moléculaire calculée: 400 aa, 43 kDa
• Poids moléculaire observé: 115 kDa
• Numéro d’acquisition GenBank: BC012350
• Symbole du gène: SPN
• Identification du gène (NCBI): 6693
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD43, also named leukosialin, sialophorin, galactoglycoprotein, leukocyte sialoglycoprotein, SPN, and is a mucin-like type I transmembrane protein. In humans, it is expressed in haematopoietic cells, including T lymphocytes, monocytes, granulocytes, natural killer cells, platelets, and haematopoietic stem cells, with the exception of mature erythrocytes and B cell subpopulations. The unglycosylated form of CD43 migrates on SDS-PAGE with a molecular weight of 54 kDa (PMID: 2943740), whereas CD43 from different haematopoietic cell lines displays increased molecular weights since it is glycosylated with O-linked chains that differ in core structure and sialylation.

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