Anticorps Polyclonal de lapin anti-CD40L/CD154

CD40L/CD154 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 16668-1-AP

Synonymes

CD154, CD40 L, CD40 ligand, CD40L, CD40L/CD154, CD40LG, gp39, hCD40L, HIGM1, IGM, IMD3, T BAM, T cell antigen Gp39, TNF related activation protein, TNFSF5, TRAP

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Galerie de données de validation

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  WB analysis of mouse small intestine using 16668-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 16668-1-AP (CD40LG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 16668-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16668-1-AP (CD40LG antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16668-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16668-1-AP (CD40L; CD154 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 16668-1-AP

  IP result of anti-CD40L/CD154 (IP:16668-1-AP, 4ug; Detection:16668-1-AP 1:1000) with Jurkat cells lysate 3200ug.

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  IHC staining of human spleen using 16668-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 16668-1-AP (CD154 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human spleen using 16668-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 16668-1-AP (CD154 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of human lung cancer using 16668-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung cancer tissue using CD40L/CD154 antibody (16668-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human lung cancer using 16668-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung cancer tissue using CD40L/CD154 antibody (16668-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu d'intestin grêle de souris, cellules HeLa, cellules Jurkat
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu splénique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cancer du poumon humain,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 5 publications below

Informations sur le produit

16668-1-AP cible CD40L/CD154 dans les applications de WB, IHC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD40L/CD154 Protéine recombinante Ag10147
• Nom complet: CD40 ligand
• Masse moléculaire calculée: 261 aa, 29 kDa
• Poids moléculaire observé: 29-32 kDa
• Numéro d’acquisition GenBank: BC071754
• Symbole du gène: CD40 Ligand
• Identification du gène (NCBI): 959
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33 kDa transmembrane homologue or 18 kDa soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and on a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. Recent studies have suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis.

Publications

Species	Application	Title
mouse	WB	Sci Adv; Promoting the activation of T cells with glycopolymer-modified dendritic cells by enhancing cell interactions.; Authors - Liyin Yu
mouse	IHC	J Mol Cell Cardiol; TNF-α promotes early atherosclerosis by increasing transcytosis of LDL across endothelial cells: crosstalk between NF-κB and PPAR-γ; Authors - Youzhi Zhang
mouse	IHC	Br J Pharmacol; CRP promotes atherosclerosis by increasing LDL transcytosis across endothelial cells.; Authors - Fang Bian
human	WB	Pharmacol Res; PKCβII-mediated cross-talk of TRPV1/CB2 modulates the glucocorticoid-induced osteoclast overactivity.; Authors - Giulia Bellini
rat	IHC	Sci China Life Sci; Compression loading of osteoclasts attenuated microRNA-146a-5p expression, which promotes angiogenesis by targeting adiponectin.; Authors - Yue Wang
mouse	WB	Bone; YAP1 is essential for osteoclastogenesis through a TEADs-dependent mechanism.; Authors - Liming Zhao