Anticorps Monoclonal anti-CD40

CD40 Monoclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF,ELISA

Conjugaison

Non conjugué

CloneNo.

2A8G5

N° de cat : 66965-1-Ig

Synonymes

B cell surface antigen CD40, Bp50, CD40, CD40L receptor, CDW40, p50, TNFRSF5

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Galerie de données de validation

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  WB analysis of Raji using 66965-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66965-1-Ig (CD40 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Daudi using 66965-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 66965-1-Ig (CD40 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 66965-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66965-1-Ig (CD40 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66965-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66965-1-Ig (CD40 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 66965-1-Ig

  Immunohistochemical analysis of paraffin-embedded human follicular lymphoma tissue slide using 66965-1-Ig (CD40 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 66965-1-Ig

  Immunohistochemical analysis of paraffin-embedded human follicular lymphoma tissue slide using 66965-1-Ig (CD40 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 66965-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD40 antibody (66965-1-Ig, Clone: 2A8G5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human tonsillitis using 66965-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD40 antibody (66965-1-Ig, Clone: 2A8G5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Raji, cellules Daudi
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu d'amygdalite humain,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:2500-1:10000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66965-1-Ig cible CD40 dans les applications de WB, IHC, IF,ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD40 Protéine recombinante Ag28040
• Nom complet: CD40 molecule, TNF receptor superfamily member 5
• Masse moléculaire calculée: 277 aa, 31 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC012419
• Symbole du gène: CD40
• Identification du gène (NCBI): 958
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cluster of differentiation 40 (CD40) is a costimulatory protein located on antigen presenting cells and is required for their activation. CD40 is a member of the tumor necrosis factor (TNF) receptor (TNFR) family.

What is the molecular weight of CD40?

The molecular weight of CD40 is 43 kDa.

What is the cellular localization of CD40?

CD40 can be secreted by cells or found in the cell membrane.

What is the tissue specificity of CD40?

CD40 is expressed in B cells and primary carcinoma cells but is also found in dendritic cells and macrophages (PMID: 10209159).

What is the function of CD40?

CD40 acts as a receptor for TNFSF5/CD40LG, which is expressed on activated T cells. This interaction is essential for B cell proliferation, expression of activation markers, immunoglobulin production, and isotype switching (PMID: 8809473). This interaction is also crucial for the formation of memory B cells and germinal centers, and signaling through CD40 prevents apoptosis of germinal center B cells.

What is the role of CD40 in disease?

Defects in CD40 lead to hyper-IgM immunodeficiency syndrome type 3 (HIGM3) (PMID: 11675497). This is an autosomal recessive disorder that includes the inability of B cells to undergo isotype switching, a key step in the final differentiation of the humoral immune response, and an inability to mount an antibody-specific immune response.