Anticorps Monoclonal anti-CD3

CD3 Monoclonal Antibody for IF

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

IF

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

3F3A1

N° de cat : CL647-60181

Synonymes

CD3E, T3E, TCRE

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Galerie de données de validation

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  IF Staining of human colon cancer using CL647-60181

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488 pan-keratin antibody (CL488-26411) at dilution of 1:200, CoraLite® Plus 647 CD3 antibody (CL647-60181, Clone: 3F3A1, Magenta) at dilution of 1:100, CoraLite®594 CD8 antibody (CL594-66868, Clone: 1G2B10, Red ) at dilution of 1:400. DAPI (Blue).

• 

  IF Staining of human colon cancer using CL647-60181

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488 pan-keratin antibody (CL488-26411) at dilution of 1:200, CoraLite® Plus 647 CD3 antibody (CL647-60181, Clone: 3F3A1, Cyan) at dilution of 1:100, CoraLite®594 CD8 antibody (CL594-66868, Clone: 1G2B10, Red ) at dilution of 1:400. DAPI (Blue).

• 

  IF Staining of human colon cancer using CL647-60181

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 647 CD3 antibody (CL647-60181, Clone: 3F3A1 ) at dilution of 1:200, CoraLite®594 CD8 antibody (CL594-66868, Clone: 1G2B10, red), CoraLite® Plus 488 pan-keratin antibody (CL488-26411, green).

• 

  IF Staining of human liver cancer using CL647-60181

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite® Plus 647 CD3 antibody (CL647-60181, Clone: 3F3A1 ) at dilution of 1:100, CoraLite®488 CD163 antibody (CL488-16646, green), CoraLite®555 Beta Catenin antibody (CL555-51067, orange).

• 

  IF Staining of human tonsillitis using CL647-60181

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 647 CD3 antibody (CL647-60181, Clone: 3F3A1 ) at dilution of 1:100.

• 

  IF Staining of human tonsillitis using CL647-60181

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 647 CD3 antibody (CL647-60181, Clone: 3F3A1 ) at dilution of 1:100.

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Applications testées

• Résultats positifs en IF: tissu de cancer du côlon humain, tissu d'amygdalite humain, tissu de cancer du foie humain

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-60181 cible CD3 dans les applications de IF et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD3 Protéine recombinante Ag11797
• Nom complet: CD3e molecule, epsilon (CD3-TCR complex)
• Masse moléculaire calculée: 207 aa, 23 kDa
• Poids moléculaire observé: 23 kDa
• Numéro d’acquisition GenBank: BC049847
• Symbole du gène: CD3E
• Identification du gène (NCBI): 916
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. This anti-CD3 is a monoclonal antibody directed against the epsilon chain of human CD3 molecule.