Anticorps Polyclonal de lapin anti-B7-H3/CD276

• Phare
• Validé par KD/KO

B7-H3/CD276 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 14453-1-AP

Synonymes

4Ig B7 H3, B7 homolog 3, B7-H3, CD276, CD276 antigen, CD276 molecule, Costimulatory molecule, UNQ309/PRO352

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Galerie de données de validation

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  WB analysis of HEK-293 using 14453-1-AP

  WB result of B7-H3/CD276 antibody (14453-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-B7-H3/CD276 transfected HEK-293 cells.

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  WB analysis using 14453-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14453-1-AP (B7-H3/CD276 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 14453-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14453-1-AP (B7-H3/CD276 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 14453-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14453-1-AP (B7-H3/CD276 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 14453-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14453-1-AP (B7-H3/CD276 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 14453-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14453-1-AP (B7-H3/CD276 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human medulloblastoma using 14453-1-AP

  Immunohistochemical analysis of paraffin-embedded human medulloblastoma using 14453-1-AP (B7-H3/CD276 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human medulloblastoma using 14453-1-AP

  Immunohistochemical analysis of paraffin-embedded human medulloblastoma using 14453-1-AP (B7-H3/CD276 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 14453-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14453-1-AP (B7-H3/CD276 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 14453-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14453-1-AP (B7-H3/CD276 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules LNCaP, cellules U2OS
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu d'amygdalite humain, tissu de médulloblastome humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 7 publications below
• IF: See 2 publications below

Informations sur le produit

14453-1-AP cible B7-H3/CD276 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: B7-H3/CD276 Protéine recombinante Ag6006
• Nom complet: CD276 molecule
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC062581
• Symbole du gène: CD276
• Identification du gène (NCBI): 80381
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

B7-H3 (CD276) is a type I transmembrane protein expressed on many tissues and cell types. B7-H3 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor (PMID: 25567370; 20696859). Overexpressed on a wide range of human solid cancers, B7-H3 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy (PMID: 27208063).

Publications

Species	Application	Title
human	WB	J Nutr Biochem; Isothiocyanates attenuate immune checkpoint blockage therapy in gastric cancer via induction of PD-L1 expression; Authors - Qi Zhang
human	WB	J Cell Physiol; Downregulated miR-187 contributes to the keratinocytes hyperproliferation in psoriasis.; Authors - Lipeng Tang
human	IHC	CNS Neurosci Ther; PTX3 mediates the infiltration, migration, and inflammation-resolving-polarization of macrophages in glioblastoma.; Authors - Hao Zhang
human	IHC	J Cancer; The immunoregulatory protein B7-H3 promotes aerobic glycolysis in oral squamous carcinoma via PI3K/Akt/mTOR pathway.; Authors - Zhangao Li
human	IHC	Med Oncol; Overexpression of B7-H3 in CD14+ monocytes is associated with renal cell carcinoma progression.; Authors - Miao Li
human	WB	J Oncol; Alpha-Fetoprotein Regulates the Expression of Immune-Related Proteins through the NF-κB (P65) Pathway in Hepatocellular Carcinoma Cells.; Authors - Qiu-Ting Li