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CD24 Polyclonal antibody

CD24 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, ELISA

CD24A

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 
SKU: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inDaudi cells, mouse brain tissue, Raji cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10600-1-AP targets CD24 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0654

Product name: Recombinant human CD24 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-80 aa of BC007674

Sequence: MGRAMVARLGLGLLLLALLLPTQIYSSETTTGTSSNSSQSTSNSGLAPNPTNATTKAAGGALQSTASLFVVSLSLLHLYS

Predict reactive species
Full Name CD24 molecule
Calculated Molecular Weight8 kDa
Observed Molecular Weight 30-70 kDa
GenBank Accession NumberBC007674
Gene Symbol CD24
Gene ID (NCBI) 100133941
RRIDAB_10646440
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP25063
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD24 (known as heat stable antigen) is a small highly glycosylated GPI-linked sialoprotein. It is normally expressed at the surface of most B lymphocytes and differentiating neuroblasts, and it is also up-regulated in a wide variety of cancers. Studies have shown that CD24 functions in the regulation of B-cell apoptosis, leukocyte signal transduction, and leukocyte adhesion. Since it is highly glycosylated, the apparent molecular weight of CD24 could be variable, ranging from 30 kDa to 70 kDa. (Ref: Akihiko Sano, MD., 2009)

Protocols

Product Specific Protocols
WB protocol for CD24 antibody 10600-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Anti-CD24 antibody (Cat# 10600-1-AP) has accumulated 26 citations published in journals including Nature Microbiology, Nature Communications, Advanced Science, Cancer Letters, International Journal of Biological Sciences, Apoptosis, eLife, and BMC Cancer. Associated research fields span oncology (breast, colorectal, esophageal, melanoma, cervical, pancreatic, lung, and kidney cancers), tumor metastasis, cancer stemness, immune microenvironment, infectious disease, neuroscience, and regenerative medicine.

SpeciesApplicationTitle
mouseIF

Nat Microbiol

Uropathogenic Escherichia coli invade luminal prostate cells via FimH-PPAP receptor binding.

Authors - Maria Guedes
humanIF

Nat Commun

Natural photosynthetic system for restoring homeostasis of animal organelle interaction network.

Authors - Chen Xia
human

Exp Mol Med

SLC38A4 promotes Kupffer cell phagocytosis and suppresses tumor liver metastasis.

Authors - Jie Li
human

Adv Sci (Weinh)

A Novel Long Non-Coding RNA lnc030 Maintains Breast Cancer Stem Cell Stemness by Stabilizing SQLE mRNA and Increasing Cholesterol Synthesis.

Authors - Yilu Qin
humanIF

Adv Sci (Weinh)

Single‐Cell Transcriptome Profiling Reveals Multicellular Ecosystem of Nucleus Pulposus during Degeneration Progression

Authors - Ji Tu
humanWB

Cancer Lett

CD24 promotes metastasis and chemoresistance by directly targeting Arf6-ERK pathway in esophageal squamous cell carcinoma.

Authors - Pan Hong
  • KD Validated
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