Tested Applications
| Positive WB detected in | Daudi cells, mouse brain tissue, Raji cells, Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10600-1-AP targets CD24 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0654 Product name: Recombinant human CD24 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-80 aa of BC007674 Sequence: MGRAMVARLGLGLLLLALLLPTQIYSSETTTGTSSNSSQSTSNSGLAPNPTNATTKAAGGALQSTASLFVVSLSLLHLYS Predict reactive species |
| Full Name | CD24 molecule |
| Calculated Molecular Weight | 8 kDa |
| Observed Molecular Weight | 30-70 kDa |
| GenBank Accession Number | BC007674 |
| Gene Symbol | CD24 |
| Gene ID (NCBI) | 100133941 |
| RRID | AB_10646440 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P25063 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD24 (known as heat stable antigen) is a small highly glycosylated GPI-linked sialoprotein. It is normally expressed at the surface of most B lymphocytes and differentiating neuroblasts, and it is also up-regulated in a wide variety of cancers. Studies have shown that CD24 functions in the regulation of B-cell apoptosis, leukocyte signal transduction, and leukocyte adhesion. Since it is highly glycosylated, the apparent molecular weight of CD24 could be variable, ranging from 30 kDa to 70 kDa. (Ref: Akihiko Sano, MD., 2009)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD24 antibody 10600-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-CD24 antibody (Cat# 10600-1-AP) has accumulated 26 citations published in journals including Nature Microbiology, Nature Communications, Advanced Science, Cancer Letters, International Journal of Biological Sciences, Apoptosis, eLife, and BMC Cancer. Associated research fields span oncology (breast, colorectal, esophageal, melanoma, cervical, pancreatic, lung, and kidney cancers), tumor metastasis, cancer stemness, immune microenvironment, infectious disease, neuroscience, and regenerative medicine.
| Species | Application | Title |
|---|---|---|
Nat Microbiol Uropathogenic Escherichia coli invade luminal prostate cells via FimH-PPAP receptor binding. | ||
Nat Commun Natural photosynthetic system for restoring homeostasis of animal organelle interaction network. | ||
Exp Mol Med SLC38A4 promotes Kupffer cell phagocytosis and suppresses tumor liver metastasis. | ||
Adv Sci (Weinh) A Novel Long Non-Coding RNA lnc030 Maintains Breast Cancer Stem Cell Stemness by Stabilizing SQLE mRNA and Increasing Cholesterol Synthesis. | ||
Adv Sci (Weinh) Single‐Cell Transcriptome Profiling Reveals Multicellular Ecosystem of Nucleus Pulposus during Degeneration Progression | ||
Cancer Lett CD24 promotes metastasis and chemoresistance by directly targeting Arf6-ERK pathway in esophageal squamous cell carcinoma.
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