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Anticorps Polyclonal de lapin anti-CD109

CD109 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 18715-1-AP

Synonymes

CD109, CD109 antigen, CD109 molecule, CPAMD7, p180, Platelet specific Gov antigen, r150, RP11 525G3.1

Galerie de données de validation

Filter:
  • Western Blot (WB) analysis of PC-3 cells using CD109 Polyclonal antibody (18715-1-AP)

    WB analysis of PC-3 using 18715-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 18715-1-AP (CD109 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of PC-3 cells using CD109 Polyclonal antibody (18715-1-AP)

    IP experiment of PC-3 using 18715-1-AP

    IP result of anti-CD109 (IP:18715-1-AP, 4ug; Detection:18715-1-AP 1:600) with PC-3 cells lysate 5000ug.

Applications testées

Résultats positifs en WBcellules PC-3,
Résultats positifs en IPcellules PC-3,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

WBSee 1 publications below

Informations sur le produit

18715-1-AP cible CD109 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Réactivité citéeHumain
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Peptide
Nom complet CD109 molecule
Masse moléculaire calculée 162 kDa
Numéro d’acquisition GenBankBC152996
Symbole du gène CD109
Identification du gène (NCBI) 135228
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD109, also named as CPAMD7, is a member of the alpha2-macroglobulin/complement superfamily. It is a glycosylphosphatidylinositol (GPI)-linked cell surface glycoprotein of approximately 170kd which is found on the cell surface of platelets, activated T-cells, and endothelial cells. CD109 binds to and negatively regulates signaling of transforming growth factor beta (TGF-beta). CD109 has been identified as part of the TGF-beta receptor system in human keratinocytes. Up regulation of CD109 expression has been observed in several different types of tumor.CD109 is a glycosyl-phosphatidylinositol-anchored glycoprotein that is a member of the a2-macroglobulin/C3, C4, C5 family of thioester-containing proteins. There're 2 forms of 150 (p150) and 120 kDa (p120) exist due to proteolytic degradation from a 180 kDa form. The antibody can recognize all these 4 isoforms.

Publications

SpeciesApplicationTitle
humanWB

Oncol Lett

CD109 expression is upregulated in penile squamous cell carcinoma.

Authors - Fengyun Dong
View Article