Anticorps Polyclonal de lapin anti-CCT7

CCT7 Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 15994-1-AP

Synonymes

CCT ETA, CCT7, Ccth, HIV 1 Nef interacting protein, Nip7 1, TCP 1 eta

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Galerie de données de validation

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  WB analysis of HeLa using 15994-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15994-1-AP (CCT7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15994-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15994-1-AP (CCT7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 15994-1-AP

  IP result of anti-CCT7 (IP:15994-1-AP, 4ug; Detection:15994-1-AP 1:1000) with HEK-293 cells lysate 2400ug.

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  IF Staining of MCF-7 using 15994-1-AP

  Immunofluorescent analysis of MCF-7 cells, using CCT7 antibody 15994-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu cérébral humain
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IF/ICC: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

15994-1-AP cible CCT7 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CCT7 Protéine recombinante Ag8868
• Nom complet: chaperonin containing TCP1, subunit 7 (eta)
• Masse moléculaire calculée: 543 aa, 59 kDa
• Poids moléculaire observé: 59 kDa
• Numéro d’acquisition GenBank: BC019296
• Symbole du gène: CCT7
• Identification du gène (NCBI): 10574
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CCT7 is a subunit of CCT complex-a large cytosolic chaperonin complex composed of two hetero-oligomeric stacked rings able to interact with nascent polypeptides, which mediates protein folding in an ATP-dependent manner and prevents aggregation in eukaryotes. Each ring consists of eight different subunits (CCT1 to CCT8). CCT7 is involved in preventing aggregation of GPCRs and in regulating their expression, maturation, and transport to the cell surface.

Publications

Species	Application	Title
human	WB	Cell; CCT2 is an aggrephagy receptor for clearance of solid protein aggregates.; Authors - Xinyu Ma
	WB	Nat Commun; Tick extracellular vesicles enable arthropod feeding and promote distinct outcomes of bacterial infection.; Authors - Adela S Oliva Chávez
human	IHC	Aging (Albany NY); Overexpression of chaperonin containing TCP1 subunit 7 has diagnostic and prognostic value for hepatocellular carcinoma.; Authors - Xinghua Huang
mouse	WB	Virology; Murine cytomegalovirus M72 promotes acute virus replication in vivo and is a substrate of the TRiC/CCT complex.; Authors - Sandhya Gopal
human	WB	Nat Commun; Purification of cross-linked RNA-protein complexes by phenol-toluol extraction.; Authors - Erika C Urdaneta
human	WB	Clin Proteomics; Quantitative proteomics identified circulating biomarkers in lung adenocarcinoma diagnosis; Authors - Hongyu Chen