Anticorps Polyclonal de lapin anti-CCT3

• Phare
• Validé par KD/KO

CCT3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(17)

N° de cat : 10571-1-AP

Synonymes

TCP 1 gamma, CCT gamma, CCTG, CCTgamma, CCT-gamma

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Galerie de données de validation

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  WB analysis using 10571-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10571-1-AP (CCT3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10571-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10571-1-AP (CCT3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 10571-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10571-1-AP (CCT3 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat kidney using 10571-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 10571-1-AP (CCT3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10571-1-AP

  IP result of anti-CCT3 (IP:10571-1-AP, 4ug; Detection:10571-1-AP 1:15000) with mouse brain tissue lysate 1720 ug.

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  IHC staining of mouse liver using 10571-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10571-1-AP (CCT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 10571-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CCT3 antibody (10571-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  IF Staining of HepG2 using 10571-1-AP

  Immunofluorescent analysis of HepG2 cells, using CCT3 antibody 10571-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  FC experiment of HepG2 using 10571-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CCT3 Polyclonal antibody (10571-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules Jurkat, cellules U2OS, tissu cérébral de rat, tissu cérébral de souris, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu hépatique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 14 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Informations sur le produit

10571-1-AP cible CCT3 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CCT3 Protéine recombinante Ag0843
• Nom complet: chaperonin containing TCP1, subunit 3 (gamma)
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC006501
• Symbole du gène: CCT3
• Identification du gène (NCBI): 7203
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Nat Genet; DYX1C1 is required for axonemal dynein assembly and ciliary motility.; Authors - Tarkar Aarti A
mouse	WB	Cell; Kinetic Analysis of Protein Stability Reveals Age-Dependent Degradation.; Authors - Erik McShane
	WB,IF	Adv Sci (Weinh); Primate-Specific DAZ Regulates Translation of Cell Proliferation-Related mRNAs and is Essential for Maintenance of Spermatogonia; Authors - Ningjing Ou
mouse,human	WB	Proc Natl Acad Sci U S A; BBS6, BBS10, and BBS12 form a complex with CCT/TRiC family chaperonins and mediate BBSome assembly.; Authors - Seo Seongjin S ; KD Validated
human	WB	J Cell Biol; CEP55 promotes cilia disassembly through stabilizing Aurora A kinase.; Authors - Yu-Cheng Zhang
human	WB,IF	Dev Biol; Involvement of multimeric protein complexes in mediating the capacitation-dependent binding of human spermatozoa to homologous zonae pellucidae.; Authors - Redgrove Kate A KA

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