Anticorps Monoclonal anti-CCRL2

CCRL2 Monoclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

CloneNo.

2C1E11

N° de cat : 66611-1-Ig

Synonymes

C C chemokine receptor like 2, CCR11, CCR6, CCRL2, Chemokine receptor CCR11, Chemokine receptor X, CKRX, CRAM, CRAM A, CRAM B, HCR

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Galerie de données de validation

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  WB analysis of HeLa using 66611-1-Ig

  HeLa cells and A431 cells were subjected to SDS PAGE followed by western blot with 66611-1-Ig (CCRL2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 66611-1-Ig

  A431 cells and K-562 cells were subjected to SDS PAGE followed by western blot with 66611-1-Ig (CCRL2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human spleen using 66611-1-Ig

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66611-1-Ig (CCRL2 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human spleen using 66611-1-Ig

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66611-1-Ig (CCRL2 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules K-562
• Résultats positifs en IHC: tissu splénique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

66611-1-Ig cible CCRL2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CCRL2 Protéine recombinante Ag4045
• Nom complet: chemokine (C-C motif) receptor-like 2
• Masse moléculaire calculée: 344 aa, 40 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC025717
• Symbole du gène: CCRL2
• Identification du gène (NCBI): 9034
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Chemokine CC motif receptor-like 2 (CCRL2) is a seven-transmembrane domain receptor that shares structural and functional similarities with the family of atypical chemokine receptors (ACKRs) (PMID: 28743719). CCRL2 does not appear to be a signaling receptor, but may have a role in modulating chemokine-triggered immune responses by capturing and internalizing CCL19 or by presenting RARRES2 ligand to CMKLR1, a functional signaling receptors. CCRL2 has been found on almost all hemopoietic cells, including monocytes, macrophages, polymorphonuclear neutrophils (PMNs), T cells, dendritic cells, natural killer cells, and CD34+ progenitor cells, with PMNs expressing the highest levels (PMID: 15188357). CCRL2 is also expressed by barrier cells, such as lymphatic and blood endothelial cells and bronchial and intestinal epithelial cells (PMID: 29056935).

Publications

Species	Application	Title
human	WB	PLoS Pathog; Identification of viral SIM-SUMO2-interaction inhibitors for treating primary effusion lymphoma.; Authors - Ling Ding
human	WB	Onco Targets Ther; Oncogenic Role and Prognostic Value of MicroRNA-937-3p in Patients with Breast Cancer.; Authors - Deyu Li
human	WB	Front Immunol; Identification and validation of chemokine system-related genes in idiopathic pulmonary fibrosis; Authors - Tianming Zhao