Anticorps Polyclonal de lapin anti-CCNL1

CCNL1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 13138-1-AP

Synonymes

ania 6a, BM 001, CCNL1, Cyclin L, cyclin L1, PRO1073, UNQ530/PRO1073

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Galerie de données de validation

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  WB analysis using 13138-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13138-1-AP (CCNL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 13138-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 13138-1-AP (CCNL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 13138-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13138-1-AP (CCNL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 13138-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13138-1-AP (CCNL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules MCF-7, cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

13138-1-AP cible CCNL1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CCNL1 Protéine recombinante Ag3731
• Nom complet: cyclin L1
• Masse moléculaire calculée: 526 aa, 60 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC007081
• Symbole du gène: CCNL1
• Identification du gène (NCBI): 57018
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CCNL1, also named as Cyclin-L1, contains a RS region and belongs to the cyclin family. CCNL1 localizes in the nucleus speckle and is Overexpressed in primary tumors of head and neck squamous cell carcinomas (HNSCC). CCNL1 as transcriptional regulator participates in regulating the pre-mRNA splicing process. It is seems to be involved in the regulation of RNA polymerase II (pol II). CCNL1 functions in association with cyclin-dependent kinases (CDKs) and has a role in the second step of splicing. It may be a candidate proto-oncogene in head and neck squamous cell carcinomas (HNSCC). The calculated molecular weight of CCNL1 is about 55-60 kDa, but we detected a 40-50 kDa isoform by western blotting.