Anticorps Polyclonal de lapin anti-CCL27

CCL27 Polyclonal Antibody for IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 22301-1-AP

Synonymes

ALP, C C motif chemokine 27, CC chemokine ILC, CCL27, CTACK, CTAK, ESKINE, IL 11 R alpha locus chemokine, ILC, PESKY, SCYA27, Skinkine, Small inducible cytokine A27

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Galerie de données de validation

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  IHC staining of human testis using 22301-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 22301-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 22301-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 22301-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 22301-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 22301-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 22301-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 22301-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 22301-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 22301-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 22301-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 22301-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin cancer using 22301-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin cancer using 22301-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 22301-1-AP (CCL27 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A431 using 22301-1-AP

  Immunofluorescent analysis of A431 cells using 22301-1-AP (CCL27 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A431 using 22301-1-AP

  Immunofluorescent analysis of A431 cells using 22301-1-AP (CCL27 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en IHC: tissu testiculaire humain, tissu cutané humain, tissu de cancer de la peau humain, tissu de cancer du foie humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

22301-1-AP cible CCL27 dans les applications de IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: chemokine (C-C motif) ligand 27
• Masse moléculaire calculée: 13 kDa
• Numéro d’acquisition GenBank: NM_006664
• Symbole du gène: CCL27
• Identification du gène (NCBI): 10850
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CCL27, also known as CTACK, is a member of the CC chemokine subfamily. CCL27 is predominantly expressed in skin and selectively attracts cutaneous lymphocyte antigen-positive T cells and Langerhans cells. The CCL27 secreted from keratinocytes was reportedly involved in inflammatory skin diseases such as atopic dermatitis, contact dermatitis, and psoriasis. CCL27 is a ligand to CCR10. The binding of CCL27 to CCR10 on T cells promotes their migration to the skin, where they can then participate in local immune responses.