Anticorps Polyclonal de lapin anti-MIP-3 Beta

MIP-3 Beta Polyclonal Antibody for WB, IF, ELISA, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 13397-1-AP

Synonymes

Beta chemokine exodus 3, C C motif chemokine 19, CCL19, CK beta 11, CKb11, EBI1 ligand chemokine, ELC, MIP 3 beta, MIP 3b, MIP-3 Beta, MIP3B, MIP-3-beta, SCYA19, Small inducible cytokine A19

---

Galerie de données de validation

• 

  WB analysis using 13397-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 13397-1-AP (MIP-3 Beta antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A549 using 13397-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using MIP-3 Beta antibody (13397-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of A549 using 13397-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 13397-1-AP (MIP-3-beta antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A549 using 13397-1-AP

  Immunofluorescent analysis of A549 cells using 13397-1-AP (MIP-3-beta antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of A549 using 13397-1-AP

  1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human MIP-3 Beta (13397-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en WB: cellules THP-1,
• Résultats positifs en IF/ICC: cellules A549,
• Résultats positifs en FC (Intra): cellules A549
• Résultats positifs en cytométrie: cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 3 publications below
• IF: See 4 publications below

Informations sur le produit

13397-1-AP cible MIP-3 Beta dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MIP-3 Beta Protéine recombinante Ag4200
• Nom complet: chemokine (C-C motif) ligand 19
• Masse moléculaire calculée: 98 aa, 12 kDa
• Numéro d’acquisition GenBank: BC027968
• Symbole du gène: CCL19/MIP-3 beta
• Identification du gène (NCBI): 6363
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CCL19, also known as MIP-3β, is one of several CC cytokine genes clustered on the p-arm of chromosome 9. CCL19 may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs. It specifically binds to chemokine receptor CCR7.

Publications

Species	Application	Title
human	IF	Am J Respir Crit Care Med; Pulmonary lymphoid neogenesis in idiopathic pulmonary arterial hypertension.; Authors - Perros Frédéric F
human	IF	Ann Rheum Dis; Global chemokine expression in systemic sclerosis (SSc): CCL19 expression correlates with vascular inflammation in SSc skin.; Authors - Allison L Mathes
human	IF	Front Immunol; The Impact of Immune Microenvironment on the Prognosis of Pancreatic Ductal Adenocarcinoma Based on Multi-Omics Analysis; Authors - Bing Yang
human	IHC	J Innate Immun; The Gene Signature of Activated M-CSF-Primed Human Monocyte-Derived Macrophages Is IL-10-Dependent; Authors - Víctor D. Cuevas
human	IHC	Front Physiol; Potential biomarkers and immune cell infiltration involved in aortic valve calcification identified through integrated bioinformatics analysis; Authors - Xiaoshuo Lv
human	IHC	Front Oncol; CXCL12, a potential modulator of tumor immune microenvironment (TIME) of bladder cancer: From a comprehensive analysis of TCGA database; Authors - Jinyan Wang