Anticorps Polyclonal de lapin anti-CEP89, CCDC123

CEP89, CCDC123 Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 24002-1-AP

Synonymes

CCDC123, CEP123, CEP89

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Galerie de données de validation

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  WB analysis of SH-SY5Y using 24002-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 24002-1-AP (CEP89 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 24002-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 24002-1-AP (CEP89 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 24002-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 24002-1-AP (CEP89 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of U-251 using 24002-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using 24002-1-AP (CEP89 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules SH-SY5Y, cellules Neuro-2a
• Résultats positifs en IF/ICC: cellules U-251,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 3 publications below

Informations sur le produit

24002-1-AP cible CEP89, CCDC123 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CEP89, CCDC123 Protéine recombinante Ag21206
• Nom complet: coiled-coil domain containing 123
• Masse moléculaire calculée: 783 aa, 90 kDa
• Poids moléculaire observé: 85-100 kDa, 40 kDa
• Numéro d’acquisition GenBank: BC136328
• Symbole du gène: CEP89
• Identification du gène (NCBI): 84902
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CCDC123(as known as CEP123), also named as CEP89, is a new player in the process of primary ciliogenesis and it also plays a role in mitochondrial metabolism where it may modulate complex IV activity. It has been shown that CEP123 is localized to the distal appendages of the mother centriolecep and the localization of CEP123 is cell cycle-dependent with its levels decreasing during mitosis. CEP123 depletion can cause defects in ciliary vesicle formationcep and prevent the formation of a ciliary vesicle at the distal end of the mother centriole. It is possible that CEP123 is involved in regulating the recruitment of membranes to the centrosome through its interaction with Cep290(PMID:23575228, 23789104, 23348840). 24002-1-AP antibody recognizes all of CEP123 isoforms.

Publications

Species	Application	Title
mouse	WB,IF	Cell Res; NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis.; Authors - Min Liu
human	IF	Dev Cell; The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base.; Authors - Tomoharu Kanie
human	IF	PLoS Biol; The evolutionary conserved proteins CEP90, FOPNL, and OFD1 recruit centriolar distal appendage proteins to initiate their assembly; Authors - Pierrick Le Borgne